Arabidopsis ZINC FINGER PROTEIN1 Acts Downstream of GL2 to Repress Root Hair Initiation and Elongation by Directly Suppressing bHLH Genes[OPEN]
Arabidopsis ZINC FINGER PROTEIN1 Acts Downstream of GL2 to Repress Root Hair Initiation and Elongation by Directly Suppressing bHLH Genes[OPEN]
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DOI：
10.1105/tpc.19.00226
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发表时间：
2020-01-01
期刊：
影响因子：
11.6
通讯作者：
Wang, Baoshan
中科院分区：
文献类型：
--
作者：
Han, Guoliang;Wei, Xiaocen;Wang, Baoshan
含有 EAR 基序的 C2H2 型锌指蛋白基因通过抑制已知在根毛起始和伸长中起重要作用的基因的表达来抑制根毛起始和伸长。Cys2His2 样折叠组 (C2H2) 型锌指蛋白通过调节其靶基因来促进根毛生长和发育。然而，人们对它们在根毛萌生和伸长中潜在的负面作用知之甚少。在这里，我们发现名为 ZINC FINGER PROTEIN1 (AtZP1) 的 C2H2 型锌指蛋白含有 ERF 相关的两亲性抑制 (EAR) 基序，可负调控拟南芥 (Arabidopsis thaliana) 根毛的起始和伸长。我们的结果表明 AtZP1 在根毛中高度表达，并且 AtZP1 在根毛发育过程中抑制转录活性。过表达AtZP1的植物缺乏根毛，而功能缺失突变体比野生型具有更长、更多的根毛。转录组分析表明，AtZP1 下调编码与根毛细胞分化和伸长相关的碱性螺旋-环-螺旋 (bHLH) 转录因子的基因。 EAR基序的突变或缺失显着降低了AtZP1的抑制活性。染色质免疫沉淀试验、AtZP1：糖皮质激素受体（GR）诱导实验、电泳迁移率变动试验和酵母单杂交试验表明，AtZP1直接靶向bHLH转录因子基因的启动子，包括关键的根毛起始基因ROOT HAIR DEFECTIVE6（RHD6）和根毛伸长基因ROOT HAIR DEFECTIVE 6-LIKE 2（RSL2） 和RSL4，并抑制根毛发育。我们的研究结果表明，AtZP1 在 GL2 下游发挥作用，通过 GL2/ZP1/RSL 途径抑制 RHD6、RSL4 和 RSL2 转录，从而负向调节根毛起始和伸长。
A C2H2-type zinc finger protein gene containing an EAR motif inhibits root hair initiation and elongation by suppressing the expression of genes known to play important roles in root hair initiation and elongation.Cys2His2-like fold group (C2H2)-type zinc finger proteins promote root hair growth and development by regulating their target genes. However, little is known about their potential negative roles in root hair initiation and elongation. Here, we show that the C2H2-type zinc finger protein named ZINC FINGER PROTEIN1 (AtZP1), which contains an ERF-associated amphiphilic repression (EAR) motif, negatively regulates Arabidopsis (Arabidopsis thaliana) root hair initiation and elongation. Our results demonstrate that AtZP1 is highly expressed in root hairs and that AtZP1 inhibits transcriptional activity during root hair development. Plants overexpressing AtZP1 lacked root hairs, while loss-of-function mutants had longer and more numerous root hairs than the wild type. Transcriptome analysis indicated that AtZP1 downregulates genes encoding basic helix-loop-helix (bHLH) transcription factors associated with root hair cell differentiation and elongation. Mutation or deletion of the EAR motif substantially reduced the inhibitory activity of AtZP1. Chromatin immunoprecipitation assays, AtZP1:glucocorticoid receptor (GR) induction experiments, electrophoretic mobility shift assays, and yeast one-hybrid assays showed that AtZP1 directly targets the promoters of bHLH transcription factor genes, including the key root hair initiation gene ROOT HAIR DEFECTIVE6 (RHD6) and root hair elongation genes ROOT HAIR DEFECTIVE 6-LIKE 2 (RSL2) and RSL4, and suppresses root hair development. Our findings suggest that AtZP1 functions downstream of GL2 and negatively regulates root hair initiation and elongation, by suppressing RHD6, RSL4, and RSL2 transcription via the GL2/ZP1/RSL pathway.