Bioactivity evaluation of porous TiO2 surface formed on titanium in mixed electrolyte by spark anodization
Bioactivity evaluation of porous TiO2 surface formed on titanium in mixed electrolyte by spark anodization
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DOI：
10.1016/j.surfcoat.2013.08.051
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发表时间：
2013-11-25
影响因子：
5.4
通讯作者：
Brantley, W. A.
中科院分区：
文献类型：
--
作者：
Park, Tae-Eon;Choe, Han-Cheol;Brantley, W. A.
本文研究了混合电解液中钛表面火花阳极氧化形成的多孔二氧化钛表面的生物活性。为了在钛上制造用于植入物应用的几何微孔，在由1.5 M H2SO 4 + 0.7 M H3 PO 4 + 0.4 M H2 O2组成的电解质中进行等离子体电解氧化（PEO）。采用FE-SEM、EDS和XPS等手段对微孔钛层的表面特征进行了研究，并通过MOT 3-E1细胞培养、细胞活力、ALP活性和体内免疫反应等方法对微孔钛层的生物活性进行了评价。随着阳极氧化时间的增加，微孔TiO 2表面的细胞粘附、增殖和存活率均高于金属钛对照组。在几何微孔表面培养的细胞具有较高的ALP活性。皮下组织和结缔组织在10分钟和20分钟后很好地附着在阳极氧化表面上。特别是，在30 mm后的微孔形成的表面上，在相邻的微孔之间观察到大量的皮下组织和结缔组织。(C)2013爱思唯尔有限公司版权所有。
In this study, the bioactivity of porous TiO2 surfaces formed on titanium in mixed electrolyte by spark anodization was investigated both in vitro and in vivo. To fabricate geometric micropores on titanium for implant applications, plasma electrolytic oxidation (PEO) was carried out in an electrolyte consisting of 1.5 M H2SO4 + 0.7 M H3PO4 + 0.4 M H2O2. The surface characteristics of the microporous Ti layer were investigated by FE-SEM, EDS, and XPS, and the bioactivity of PEO surfaces was assessed by MOT3-E1 cell culture, cell viability, ALP activity, and in vivo immune response.A gradual increase of the spark width and the voltage applied caused pitting that leads to the formation of circular pores distributed over the entire surface. The fraction of the surface area occupied by pores increased as the anodizing time increased.The microporous TiO2 surface provided higher cell adhesion, proliferation and viability compared to the metallic titanium control group. Cells cultured on the surface of geometrical microporosity demonstrated higher ALP activity. Hypodermic tissue and connective tissue were well attached on anodized surface after 10 and 20 min. In particular, on the micropore-formed surface after 30 mm, an abundant amount of hypodermic and connective tissue was observed among the adjacent micropores. (C) 2013 Elsevier B.V. All rights reserved.