Identification of the major protein component of the pneumococcal eclipse complex
Identification of the major protein component of the pneumococcal eclipse complex
复制标题
DOI：
10.1128/jb.00687-07
复制
发表时间：
2007-09-01
影响因子：
3.2
通讯作者：
Claverys, Jean-Pierre
中科院分区：
文献类型：
--
作者：
Morrison, Donald A.;Mortier-Barriere, Isabelle;Claverys, Jean-Pierre
在肺炎链球菌的遗传转化过程中，来自天然供体DNA的单链进入感受态细胞，在那里它们与分子量为15至20 kDa的未鉴定蛋白质结合形成eclipse复合物。使用Western印迹，我们确定了与供体DNA共分馏的主要蛋白质在此复合物中为SsbB。
During genetic transformation of Streptococcus pneumoniae, single strands from native donor DNA enter competent cells, where they associate with an unidentified protein with a molecular mass of 15 to 20 kDa to form the eclipse complex. Using Western blotting, we identify the principal protein cofractionating with donor DNA in this complex as SsbB.