A gustatory receptor tuned to D-fructose in antennal sensilla chaetica of Helicoverpa armigera
A gustatory receptor tuned to D-fructose in antennal sensilla chaetica of Helicoverpa armigera
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棉铃虫触角毛感器中调节 D-果糖的味觉受体
DOI：
10.1016/j.ibmb.2015.03.002
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发表时间：
2015-05-01
影响因子：
3.8
通讯作者：
Wang, Chen-Zhu
中科院分区：
文献类型：
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作者：
Jiang, Xiao-Jing;Ning, Chao;Wang, Chen-Zhu
昆虫味觉系统在昆虫的食物选择和取食行为中起着重要作用。尽管对果蝇味觉的研究取得了巨大的进展，但对于其他昆虫来说，味觉信号的关键元件之一味觉受体（GR）仍然是一个谜。本文报道了果糖诱导棉铃虫对果糖的行为和生理反应，并鉴定了果糖的味觉受体。使用长鼻延伸反射（PER）测定，我们发现，女性响应果糖刺激后的天线的远端部分，在那里我们已经确定了接触化学感受器调谐到果糖在尖端记录实验。我们分离出三个全长cDNA编码的候选人HarmGR的基础上比较orthogon-GR序列Heliothis virescens和功能特征的响应HarmGR 4 15化学品时，这种受体表达在异种卵母细胞与双电极电压钳记录。在测试的促味剂中，卵母细胞仅对D-果糖（EC 50 = 0.045 M）有剂量依赖性反应。这些结果为进一步研究味觉感受的分子机制提供了基础知识。(C)2015爱思唯尔有限公司版权所有。
Insect gustatory systems play important roles in food selection and feeding behaviors. In spite of the enormous progress in understanding gustation in Drosophila, for other insects one of the key elements in gustatory signaling, the gustatory receptor (GR), is still elusive. In this study, we report that fructose elicits behavioral and physiological responses in Helicoverpa armigera (Harm) to fructose and identify the gustatory receptor for this sugar. Using the proboscis extension reflex (PER) assays we found that females respond to fructose following stimulation of the distal part of the antenna, where we have identified contact chemosensilla tuned to fructose in tip recording experiments. We isolated three full-length cDNAs encoding candidate HarmGRs based on comparison with orthologous GR sequences in Heliothis virescens and functionally characterized the responses of HarmGR4 to 15 chemicals when this receptor was expressed in Xeno pus oocytes with two-electrode voltage-clamp recording. Among the tastants tested, the oocytes dose-dependently responded only to D-fructose (EC50 = 0.045 M). By combining behavioral, electrophysiological and molecular approaches, these results provide basic knowledge for further research on the molecular mechanisms of gustatory reception. (C) 2015 Elsevier Ltd. All rights reserved.