In situ imaging of intracellular human telomerase RNA with molecular beacon-functionalized gold nanoparticles.
In situ imaging of intracellular human telomerase RNA with molecular beacon-functionalized gold nanoparticles.
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DOI：
10.1039/d0ay00461h
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发表时间：
2020-05
期刊：
影响因子：
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通讯作者：
Tao Xie;Ziyan Fan;Ruilong Zhang;Xiaohe Tian;Guangmei Han;Zhengjie Liu;Zhongpin Zhang
中科院分区：
文献类型：
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作者：
Tao Xie;Ziyan Fan;Ruilong Zhang;Xiaohe Tian;Guangmei Han;Zhengjie Liu;Zhongpin Zhang
由于肿瘤细胞中人端粒酶RNA（human telomerase RNA，hTR）的表达水平远高于正常细胞，因此hTR的测定在肿瘤生物学研究中具有重要意义。在这项工作中，我们报告了分子信标功能化的金纳米粒子在活细胞中的hTR成像。分子信标具有带有hTR识别序列和红色荧光团Cy 5的环-茎结构。在hTR存在下，hTR序列可与分子信标的环杂交形成双链DNA链，从而使荧光状态从“关闭”转变为“打开”。在与细胞共孵育后，探针可以容易地渗透到细胞中，导致细胞内hTR的原位成像。该方法可用于区分肿瘤细胞和正常细胞，并评估不同肿瘤细胞中hTR的表达水平。此外，所提出的方法使我们能够动态监测hTR在活细胞中的表达水平，并在肿瘤诊断和hTR相关药物递送中具有巨大的应用潜力。
Since the expression level of human telomerase RNA (hTR) in tumor cells is much higher than that in normal cells, the determination of hTR is of prime importance in biological research of tumors. In this work, we report molecular beacon-functionalized gold nanoparticles for hTR imaging in live cells. The molecular beacon has a loop-and-stem structure with hTR recognition sequences and a red fluorophore Cy5. In the presence of hTR, the hTR sequence could be hybridized with the loop of molecular beacon to form a duplex DNA chain and thus the fluorescence state switched from "off" to "on". After co-incubation with cells, the probe could readily permeate into cells, leading to the in situ imaging of intracellular hTR. The proposed approach could be used to differentiate tumor cells from normal ones and assess hTR expression levels in different tumor cells. Furthermore, the proposed approach allowed us to dynamically monitor the expression level of hTR in live cells and holds great potential for application in tumor diagnosis and hTR-related drug delivery.