INVERSELY REPEATING INTEGRATED HEPATITIS-B VIRUS-DNA AND CELLULAR FLANKING SEQUENCES IN THE HUMAN HEPATOMA-DERIVED CELL-LINE HUSP
INVERSELY REPEATING INTEGRATED HEPATITIS-B VIRUS-DNA AND CELLULAR FLANKING SEQUENCES IN THE HUMAN HEPATOMA-DERIVED CELL-LINE HUSP
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DOI：
10.1073/pnas.82.1.208
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发表时间：
1985-01-01
影响因子：
11.1
通讯作者：
KOIKE, K
中科院分区：
文献类型：
--
作者：
MIZUSAWA, H;TAIRA, M;KOIKE, K
在从人肝癌衍生细胞系huSP克隆的携带整合的B型肝炎病毒（HBV）DNA序列的重组质粒中，1个克隆hu 489显示出一些不寻常的特征。该克隆插入片段的2.25-乙酰氨基转移酶Eco-D片段仅与HBV DNA探针杂交，并测定其核苷酸序列。病毒序列，以及细胞侧翼序列，表现出广泛的重排伴随着反向重复。Eco-D片段包含从S基因5“端到X基因中部的HBV DNA，随后是一个长的细胞侧翼序列。基因X的一部分在相同基因S的头部反向重复，以头对头构型被相同的细胞序列截短。在Eco-D片段的两个不同位点发现病毒DNA与细胞序列的连接序列相同，但极性相反。
Among recombinant phages carrying integrated hepatitis B virus (HBV) DNA sequences cloned from the human hepatoma-derived cell line huSP, 1 clone, .lambda.hu489, revealed some unusual features. The 2.25-kilobase Eco-D fragment from the insert of this clone hybridized to the HBV DNA probe only and its nucleotide sequence was determined. The viral sequence, as well as a cellular flanking sequence, showed extensive rearrangement accompanied by inverted repetition. The Eco-D fragment contained HBV DNA from the 5''-end region of gene S to the middle of gene X, followed by a long cellular flanking sequence. A part of the gene X was inversely repeated at the head of the same gene S in a head-to-head configuration truncated by the same cellular sequence. The same junction sequence of viral DNA and the cellular sequence was found at 2 different sites in the Eco-D fragment in opposite polarities.