Effects of cyclic stretch on proliferation of mesenchymal stem cells and their differentiation to smooth muscle cells
Effects of cyclic stretch on proliferation of mesenchymal stem cells and their differentiation to smooth muscle cells
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DOI：
10.1016/j.bbrc.2009.08.072
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发表时间：
2009-10-23
影响因子：
3.1
通讯作者：
Shokrgozar, Mohammad Ali
中科院分区：
文献类型：
--
作者：
Ghazanfari, Samane;Tafazzoli-Shadpour, Mohammad;Shokrgozar, Mohammad Ali
骨髓间充质干细胞（MSCs）能够分化为多种细胞类型，如血管平滑肌细胞（SMC）。在这项研究中，我们研究了周期性拉伸对不同加载条件下hMSCs增殖、肌动蛋白丝排列以及随后向SMC分化的影响。利用定制的装置将来自骨髓的分离细胞暴露于循环拉伸。MTT法检测细胞增殖，设计的图像处理程序检测肌动蛋白纤维的排列，荧光染色检测细胞分化。结果表明，周期性应变促进hMSCs的增殖，随着应变振幅和周期数的增加而增强。这种负载调节平滑肌α-肌动蛋白，并重新定向肌动蛋白纤维。周期性牵张导致hMSCs向SMC分化，而不添加生长因子。结论：对hMSCs进行适当的负载处理，可以提高其增殖能力，为工程组织提供功能性的平滑肌细胞。(C)2009 Elsevier Inc. All rights reserved.
Bone marrow mesenchymal stem cells (MSCs) are capable of differentiating into a variety of cell types such as vascular smooth muscle cells (SMCs). In this study, we investigated influence of cyclic stretch on proliferation of hMSCs for different loading conditions, alignment of actin filaments, and consequent differentiation to SMCs. Isolated cells from bone marrow were exposed to cyclic stretch utilizing a customized device. Cell proliferation was examined by MTT assay, alignment of actin fibers by a designed image processing code, and cell differentiation by fluorescence staining. Results indicated promoted proliferation of hMSCs by cyclic strain, enhanced by elevated strain amplitude and number of cycles. Such loading regulated smooth muscle alpha-actin, and reoriented actin fibers. Cyclic stretch led to differentiation of hMSCs to SMCs without addition of growth factor. It was concluded that applying appropriate loading treatment on hMSCs could enhance proliferation capability, and produce functional SMCs for engineered tissues. (C) 2009 Elsevier Inc. All rights reserved.