Combined detection of estrogen and tumor markers is an important reference factor in the diagnosis and prognosis of lung cancer
Combined detection of estrogen and tumor markers is an important reference factor in the diagnosis and prognosis of lung cancer
复制标题
DOI：
10.1002/jcb.27130
复制
发表时间：
2019-01-01
影响因子：
4
通讯作者：
Zhao, Jinping
中科院分区：
文献类型：
--
作者：
Bai, Yuquan;Shen, Wulin;Zhao, Jinping
肺癌肿瘤标志物与肺癌性别差异的相关性仍是一个值得进一步研究的临床问题。本研究旨在探讨17 β-雌激素（E2）与肿瘤标志物联合检测在肺癌诊断及预后判断中的意义。共入组174例患者，包括117例非小细胞肺癌（NSCLC）患者和57例良性肺部病变（BPL）患者。采用酶联免疫吸附法检测E2、癌胚抗原（CEA）、神经元特异性烯醇化酶（NSE）和细胞角蛋白19片段抗原21-1（CYFRA 21 -1）在NSCLC和BPL中的表达，分析E2与CEA、NSE和CYFRA 21 -1表达的相关性，以及与临床病理特征和预后的关系。然后在不同的肺癌细胞（A549、H1795、H460和SK-MES-1）中检查肿瘤标志物的表达。采用实时荧光定量逆转录聚合酶链反应（RT-PCR）和Western blot检测肿瘤标志物的表达。Western blot检测p-p44/42丝裂原活化蛋白激酶（MAPK）和磷酸化AKT（p-AKT）的表达。E2、CEA、NSE、CYFRA 21 -1在NSCLC患者中的表达水平显著高于BPL患者（P < .05），E2与肿瘤标志物呈正相关（P < .01）。E2和肿瘤标志物高表达的患者预后差（P <0.05）。RT-PCR和Western blot分析显示，E2组CEA、NSE、CYFRA 21 -1、p-p44/42 MAPK和p-AKT的表达水平均高于其他各组（P < .05）。这些研究表明，E2与肿瘤标志物的相互作用可显著提高肿瘤标志物在肺癌诊断和预后中的作用。
The correlation between lung cancer tumor markers and sex differences in lung cancer remains a clinical problem that is worthy of further study. This study investigated the significance of the combined detection of 17 beta-estrogen (E2) and tumor markers in the diagnosis and prognosis of lung cancer. A total of 174 patients, including 117 patients with non-small-cell lung cancer (NSCLC) and 57 patients with benign pulmonary lesions (BPL), were enrolled. An enzyme-linked immunosorbent assay was used to detect the expression of E2, carcinoembryonic antigen (CEA), neuron-specific enolase (NSE), and cytokeratin 19 fragment antigen 21-1 (CYFRA21-1) in patients with NSCLC and BPL to analyze the correlation between E2 and CEA, NSE or CYFRA21-1 expression, and its correlation with clinicopathological features and prognosis. The expression of tumor markers was then examined in different lung cancer cells (A549, H1795, H460, and SK-MES-1). The expression of tumor markers was detected by a real-time reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis. The expressions of p-p44/42 mitogen-activated protein kinase (MAPK) and phospho-AKT (p-AKT) were detected by Western blot analysis. The expression levels of E2, CEA, NSE, and CYFRA21-1 in patients with NSCLC were significantly higher than those in patients with BPL (P < .05); E2 was positively correlated with tumor markers (P < .01). Patients with a high expression of E2 and tumor markers showed a poor prognosis (P < .05). RT-quantitative PCR and Western blot analysis showed that the expression levels of CEA, NSE, CYFRA21-1, p-p44/42 MAPK, and p-AKT in the E2 group were higher than those in the other groups (P < .05). These studies indicate that the interaction of E2 and tumor markers can significantly improve the role of tumor markers in the diagnosis and prognosis of lung cancer.