From Stable to Lab-Investigating Key Factors for Sudden Deaths Caused by Streptococcus suis
From Stable to Lab-Investigating Key Factors for Sudden Deaths Caused by Streptococcus suis
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DOI：
10.3390/pathogens8040249
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发表时间：
2019-12-01
期刊：
影响因子：
3.7
通讯作者：
de Buhr, Nicole
中科院分区：
文献类型：
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作者：
Hennig-Pauka, Isabel;Imker, Rabea;de Buhr, Nicole
猪群被主要的猪病原体链球菌（Streptococcus（S.）猪。从殖民地S。存在于扁桃体中的SUIS和疾病的恶化尚未阐明。本文对某动物园在粗放式饲养条件下饲养的育肥猪的猝死现象进行了分析。这些动物突然死于感染性休克，并表现出弥散性血管内凝血病。分离的S.进行了一种扁桃体分离株和一种侵袭性CPS 2型菌株的SUIS菌株。孤立S。猪扁桃体中分离到1株猪链球菌，属于cps 2型ST 28株。从健康动物身上分离到一株属于ST 1173的猪分离株。也不是S。猪的生长、中性粒细胞胞外陷阱的诱导、血液中的存活都不能解释猝死。用动物园不同年龄组猪的血清样本进行的重组血液分析表明，个体对致病性cps 2型菌株的保护作用不同，尤其是在年轻的猪中。这些发现强调了在自体候选疫苗选择之前通过序列分型进一步表征每种情况下的致病菌株的益处。
Swine stocks are endemically infected with the major porcine pathogen Streptococcus (S.) suis. The factors governing the transition from colonizing S. suis residing in the tonsils and the exacerbation of disease have not yet been elucidated. We analyzed the sudden death of fattening pigs kept under extensive husbandry conditions in a zoo. The animals died suddenly of septic shock and showed disseminated intravascular coagulopathy. Genotypic and phenotypic characterizations of the isolated S. suis strains, a tonsillar isolate and an invasive cps type 2 strain, were conducted. Isolated S. suis from dead pigs belonged to cps type 2 strain ST28, whereas one tonsillar S. suis isolate harvested from a healthy animal belonged to ST1173. Neither S. suis growth, induction of neutrophil extracellular traps, nor survival in blood could explain the sudden deaths. Reconstituted blood assays with serum samples from pigs of different age groups from the zoo stock suggested varying protection of individuals against pathogenic cps type 2 strains especially in younger pigs. These findings highlight the benefit of further characterization of the causative strains in each case by sequence typing before autologous vaccine candidate selection.