Phylogenetic relationships among isolates of Eudorina species (Volvocales, Chlorophyta) inferred from molecular and biochemical data
Phylogenetic relationships among isolates of Eudorina species (Volvocales, Chlorophyta) inferred from molecular and biochemical data
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DOI：
10.1046/j.1529-8817.1999.3540815.x
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发表时间：
1999-08-01
影响因子：
2.9
通讯作者：
Coleman, AW
中科院分区：
文献类型：
--
作者：
Angeler, DG;Schagerl, M;Coleman, AW
通过对核糖体DNA(RDNA)重复序列的内部转录间隔区(ITS 1和ITS 2)进行测序，对代表5个种的19个菌株、1个Pleodorina inca菌株和7个Yamagishiella unicocca菌株进行了系统发育分析。序列数据确定了五个系统发育组，一个由独角耶氏菌组成，另外四个包括所有真球藻物种。两个菌株Eudorina sp.(ASW 05157)和Pleodorina INDIA(ASW 05153)的亲缘关系尚不确定，其中一个单系群仅包括E.elegans菌株，其他Eleodorina elegans菌株与圆柱藻、伊利诺氏E.illinoissis和独角藻变种一起出现。在其他Eudorina分支中的独角藻。类胡萝卜素氯黄质([3R，3‘R，6’R]β，epsilon-胡萝卜素-3，19，3‘-三醇)是一种系统有用的生化标志物，其在叶绿素鞭毛虫中的分布模式与分子数据相吻合。然而，在所推断的6个系统发育系中，它要么完全不存在，要么普遍存在，而在仅包含E，elegans分离物的E.elegans分支中随机出现。这些结果证实了目前的假说，即线虫独特的营养形态是在进化过程中的不同时期独立出现的，不是单系群的标志。
Phylogenetic analyses of 19 strains representing five species of Eudorina, one strain of Pleodorina indica, and seven strains of Yamagishiella unicocca were carried out by sequencing the internal transcribed spacer region (ITS 1 and ITS 2) of the nuclear ribosomal DNA (rDNA) repeats. The sequence data resolved five phylogenetic groups, one consisting of Y. unicocca and the other four encompassing all the Eudorina species. Two isolates, Eudorina sp, (ASW 05157) and Pleodorina indica (ASW 05153), were of uncertain affiliation, Whereas one monophyletic group included strains of E. elegans only, the other strains of E. elegans appeared alongside E. cylindrica, E. illinoisensis, and E. unicocca var. unicocca in the other Eudorina clades. The distribution pattern of the carotenoid loroxanthin ([3R,3'R,6'R]beta,epsilon-carotene-3,19,3'-triol), a systematically useful biochemical marker within chlorophycean flagellates, was shown to match the evaluated molecular data. Whereas it was either totally absent or universally present in six of the deduced phylogenetic lines, it occurred randomly in the E. elegans clade containing only E, elegans isolates. The results substantiated the current hypothesis that the unique vegetative morphology of E. elegans has independently arisen at various times during evolution and that it is not a marker of a monophyletic group.