Characterization of the rumen and fecal microbiome in bloated and non-bloated cattle grazing alfalfa pastures and subjected to bloat prevention strategies
Characterization of the rumen and fecal microbiome in bloated and non-bloated cattle grazing alfalfa pastures and subjected to bloat prevention strategies
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DOI：
10.1038/s41598-019-41017-3
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发表时间：
2019-03-12
影响因子：
4.6
通讯作者：
Khafipour, E.
中科院分区：
文献类型：
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作者：
Azad, E.;Derakhshani, H.;Khafipour, E.
泡沫性肿胀是放牧苜蓿草地的牛的一种常见的致命消化系统疾病。本研究的目的是研究与苜蓿诱导的泡沫性腹胀的发展相关的瘤胃和粪便微生物群动态，并进一步探讨腹胀预防策略如何影响这些微生物群落的组成。在3 × 3交叉实验中，12头瘤胃插管的阉牛依次经历：（1）纯苜蓿牧场，（2）补充有普朗尼克洗涤剂ALFASURE的纯苜蓿牧场，和（3）苜蓿-红豆草混合牧场。纯苜蓿牧场中12头牛中有11头出现临床腹胀，而ALFASURE治疗防止了所有12头牛中腹胀的发展，苜蓿-红豆草预防了11头牛中的5头牛中的腹胀。瘤胃内容物的微生物群分布的相当大的变化与胃胀气的发展。特别是，来自臃肿的阉牛的固体瘤胃内容物的微生物群包含更高的物种丰富度和多样性。链球菌，琥珀弧菌和未分类的粘球菌丰富的瘤胃微生物群的臃肿的公牛，而纤维杆菌和瘤胃球菌的非臃肿的公牛的瘤胃内容物中的代表性过高。我们的研究结果提供了新的见解与膨胀相关的变化的组成和预测功能特性的牛放牧苜蓿草地的瘤胃微生物群。
Frothy bloat is an often fatal digestive disorder of cattle grazing alfalfa pastures. The aim of this study was to investigate ruminal and fecal microbiota dynamics associated with development of alfalfa-induced frothy bloat and to further explore how bloat prevention strategies influence the composition of these microbial communities. In a 3 x 3 crossover experiment, twelve rumen-cannulated steers were sequentially subjected to: (1) pure alfalfa pasture, (2) pure alfalfa pasture supplemented with the pluronic detergent ALFASURE, and (3) alfalfa - sainfoin mixed pasture. Eleven out of 12 steers in pure alfalfa pasture developed clinical bloat, whereas ALFASURE treatment prevented the development of bloat in all 12 steers and alfalfa - sainfoin prevented bloat in 5 out of 11 steers. Development of bloat was associated with considerable shifts in the microbiota profile of rumen contents. In particular, the microbiota of solid rumen contents from bloated steers contained higher species richness and diversity. Streptococcus, Succinivibrio and unclassified Myxococcales were enriched in the rumen microbiota of bloated steers, whereas Fibrobacter and Ruminococcus were overrepresented in the rumen contents of non-bloated steers. Our results provide novel insights into bloat-associated shifts in the composition and predicted functional properties of the rumen microbiota of cattle grazing alfalfa pasture.