Molecular epidemiology of methicillin-resistant and methicillin-susceptible Staphylococcus aureus isolates from global clinical trials
Molecular epidemiology of methicillin-resistant and methicillin-susceptible Staphylococcus aureus isolates from global clinical trials
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DOI：
10.1128/jcm.00521-08
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发表时间：
2008-09-01
影响因子：
9.4
通讯作者：
Twynholm, Monique E.
中科院分区：
文献类型：
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作者：
Goering, Richard V.;Shawar, Ribhi M.;Twynholm, Monique E.
确定金黄色葡萄球菌的遗传特征对于更好地了解这种生物体的全球和动态流行病学非常重要，因为我们目睹了有毒和耐药克隆的出现和传播。我们对292 S进行了基因分型。金黄色葡萄球菌分离株（105甲氧西林耐药和187甲氧西林敏感）使用脉冲场凝胶电泳，多位点序列分型，SCCmec分型的组合。此外，S.测试金黄色葡萄球菌分离株中Panton-Valentine杀白细胞素（PVL）基因的存在。在瑞他帕林（一种新的局部抗生素）的5项III期全球临床试验中，从10个不同国家的无并发症皮肤感染患者中回收了分离株。最常见的耐甲氧西林克隆具有多位点序列类型8、脉冲场类型USA 300和SCCmec类型IV，并且具有PVL基因。这个克隆体只在美国被隔离。最常见的PVL阳性，甲氧西林敏感克隆具有多位点序列类型121和脉冲场类型USA 1200。这种克隆主要在南非和俄罗斯联邦发现。其他克隆被发现在较低的频率和有限的地理分布。总体而言，相当大的遗传多样性内观察到多位点序列型克隆复合体和脉冲场类型。
Determining the genetic characteristics of Staphylococcus aureus is important for better understanding of the global and dynamic epidemiology of this organism as we witness the emergence and spread of virulent and antibiotic-resistant clones. We genotyped 292 S. aureus isolates (105 methicillin resistant and 187 methicillin susceptible) using a combination of pulsed-field gel electrophoresis, multilocus sequence typing, and SCCmec typing. In addition, S. aureus isolates were tested for the presence of the Panton-Valentine leukocidin (PVL) genes. Isolates were recovered from patients with uncomplicated skin infections in 10 different countries during five phase III global clinical trials of retapamulin, a new topical antibiotic agent. The most common methicillin-resistant clone had multilocus sequence type 8, pulsed-field type USA300, and SCCmec type IV and possessed the PVL genes. This clone was isolated exclusively in the United States. The most common PVL-positive, methicillin-susceptible clone had multilocus sequence type 121 and pulsed-field type USA1200. This clone was found primarily in South Africa and the Russian Federation. Other clones were found at lower frequencies and were limited in their geographic distribution. Overall, considerable genetic diversity was observed within multilocus sequence type clonal complexes and pulsed-field types.