DIFFERENTIAL VULNERABILITY IN THE HINDBRAIN NEURONS AND LOCAL CEREBRAL BLOOD-FLOW DURING BILATERAL VERTEBRAL OCCLUSION IN GERBILS
DIFFERENTIAL VULNERABILITY IN THE HINDBRAIN NEURONS AND LOCAL CEREBRAL BLOOD-FLOW DURING BILATERAL VERTEBRAL OCCLUSION IN GERBILS
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DOI：
10.1016/0306-4522(93)90343-e
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发表时间：
1993-09-01
期刊：
影响因子：
3.3
通讯作者：
KAMADA, T
中科院分区：
文献类型：
--
作者：
HATA, R;MATSUMOTO, M;KAMADA, T
用免疫组织化学方法检测沙鼠后脑缺血时后脑神经元的差异易损性。双侧椎动脉在进入颈椎横孔前的颅外闭塞引起后脑缺血。缺血5min后，用定量放射自显影技术测定局部脑血流，小脑、脑桥、髓质的血流量降至5ml / 100g / min以下，表明闭塞后立即引起了严重的、可重复的后脑缺血。免疫组化实验选用沙鼠4只，缺血时间分别为5、10、15、30分钟。缺血5 min后，前庭外侧核和小脑间位核均可见微管相关蛋白2免疫组化病变。这些结果表明，尽管后脑不同区域的血流量明显减少，但这些区域比其他区域更脆弱。相比之下，与呼吸或心血管控制有关的区域对缺血的抵抗力较强。本研究提示后脑缺血时的选择性易损性主要取决于后脑各神经元的代谢特性不同。
Differential vulnerability in the hindbrain neurons was examined immunohistochemically during hindbrain ischemia in the gerbil. Hindbrain ischemia was produced by extracranial occlusion of the bilateral vertebral arteries just before their entry into the transverse foramen of the cervical vertebra. Local cerebral blood flow was measured by quantitative autoradiographic technique after 5 min of ischemia and was reduced to less than 5 ml/100 g per min in the cerebellum, the pons, and the medulla, indicating that severe and reproducible hindbrain ischemia was induced immediately after occlusion. For immunohistochemical investigation, four gerbils each were used for each ischemic period of 5, 10, 15, and 30 min. Immunohistochemical lesions, detected by the reaction for microtubule-associated protein 2, were visible in the lateral vestibular nucleus and the cerebellar interpositus nucleus even after 5 min of ischemia. These results suggested that these areas were more vulnerable than others, although blood flow was markedly reduced in various regions of the hindbrain. In contrast, areas related to respiratory or cardiovascular control were rather resistant to ischemia.The present study suggests that selective vulnerability during hindbrain ischemia depends mainly on different metabolic characteristics inherent to various neurons in the hindbrain.