Induction of Erythroid-Specific Expression in Murine Erythroleukemia (MEL) Cell Lines.
Induction of Erythroid-Specific Expression in Murine Erythroleukemia (MEL) Cell Lines.
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DOI：
10.1385/0-89603-178-0:421
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发表时间：
1991-01-01
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影响因子：
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通讯作者：
Antoniou, M
中科院分区：
文献类型：
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作者：
Antoniou, M
小鼠红细胞白血病（MEL）细胞系是来源于感染Friend病毒复合体1的易感小鼠脾脏的红细胞祖细胞。这些病毒转化的细胞停留在红细胞原发育阶段，可以在组织培养中无限期维持(2)。大多数MEL细胞系表现出低水平（约1%）的自发红系分化，这取决于培养条件以及所讨论的细胞系（2-4）。然而，在各种化学试剂的作用下，MEL细胞可以被诱导进行更高水平的红系分化（30-100%）。最有效的分化诱导剂包括极平面化合物(如二甲亚砜（5)和六亚甲基双乙酰胺(6)）、脂肪酸盐（如丁酸钠(7)）、视黄酸及其一些衍生物(8)(有关诱导剂的全面列表，请参阅。9)。
Murine erythroleukemia (MEL) cell lines are erythroid progenitor cells derived from the spleens of susceptible mice infected with the Friend virus complex 1. These virally transformed cells are arrested at the proerythroblast stage of development and can be maintained in tissue culture indefinitely (2). Most MEL cell lines show a low (approx 1 %) level of spontaneous erythroid differentiation, which is dependent on the culture conditions as well as the cell line in question (2-4). However, upon treatment with various chemical agents, MEL cells can be induced to undergo erythroid differentia- tion at much higher levels (30-100%). Among the most potent inducers of differentiation are polar-planar compounds (such as dimethyl sulfoxide (5) and hexamethylene bisacetamide (6), fatty acid salts (for example, sodium butyrate (7), retinoic acid and some of its derivatives (8) (for a comprehensive list of inducers, seeref. 9).