Oral biofilm elimination by combining iron-based nanozymes and hydrogen peroxide-producing bacteria
Oral biofilm elimination by combining iron-based nanozymes and hydrogen peroxide-producing bacteria
复制标题
通过结合铁基纳米酶和产生过氧化氢的细菌消除口腔生物膜
DOI：
10.1039/c9bm01889a
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发表时间：
2020
影响因子：
6.6
通讯作者：
Gao Lizeng
中科院分区：
文献类型：
--
作者：
Wang Yanqiu;Shen Xinyu;Ma Shang;Guo Qianqian;Zhang Wei;Cheng Lu;Ding Liming;Xu Zhuobin;Jiang Jing;Gao Lizeng
就许多学童和绝大多数成年人的口腔健康而言，龋齿是一种全球性风险。龋齿形成的主要因素是细菌附着在牙齿表面形成口腔生物膜，产生酸使钙脱矿，最终导致蛀牙。口腔生物膜的消除仍然是一个挑战，因为细菌嵌入内部，生物膜基质保护它们，防止抗生素或杀菌剂的渗透。已经开发出破坏口腔生物膜的有前途的策略，包括使用天然酶降解生物膜基质和使用过氧化氢杀死细菌。在这里，我们展示了一种策略，将具有过氧化物酶样活性的纳米酶与产生生物过氧化氢的细菌相结合，以消除口腔生物膜以治疗龋齿。通过使用唾液涂覆的羟基磷灰石盘和切片的人牙来模拟真实的口腔环境，我们分析了在可产生过氧化氢的戈登链球菌存在的情况下，氧化铁纳米酶或硫化铁纳米酶对变形链球菌生物膜的影响。细菌活力测定和生物膜形态表征表明，纳米酶和细菌的结合显着减少了细菌数量（减少 5 lg）和生物膜基质（减少 85%）。因此，铁基纳米酶与产生过氧化氢的细菌的结合可能为龋齿治疗中消除口腔生物膜提供新策略。
Dental caries is a global risk in terms of oral health in many schoolchildren and in a vast majority of adults. The primary factor for caries formation is the attachment of bacteria on the tooth surface to form an oral biofilm which generates acids to demineralize calcium and eventually cause tooth decay. Oral biofilm elimination is still a challenge because bacteria are embedded inside with the biofilm matrix protecting them, preventing the penetration of antibiotics or bactericides. Promising strategies for disrupting oral biofilms have been developed, including the use of natural enzymes to degrade the biofilm matrix and hydrogen peroxide to kill bacteria. Here we demonstrate a strategy that combines nanozymes with peroxidase-like activity and bacteria generating biogenic hydrogen peroxide to eliminate oral biofilms for caries treatment. By using a saliva-coated hydroxyapatite disc and sectioned human tooth to mimic the real oral environment, we analyze the influence of iron oxide nanozymes or iron sulfide nanozymes on a Streptococcus mutans biofilm in the presence of Streptococcus gordonii which can generate hydrogen peroxide. Bacterial viability assays and biofilm morphology characterization show that the combination of nanozymes and bacteria remarkably reduces the bacteria number (5 lg reduction) and biofilm matrix (85% reduction). Therefore, the combination of iron-based nanozymes and hydrogen peroxide-generating bacteria may provide a new strategy for oral biofilm elimination in dental caries treatment.