Susceptibility and intra-species transmission of the H9N2 G1 prototype lineage virus in Japanese quail and turkeys
Susceptibility and intra-species transmission of the H9N2 G1 prototype lineage virus in Japanese quail and turkeys
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DOI：
10.1016/j.vetmic.2013.03.014
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发表时间：
2013-07-26
影响因子：
3.3
通讯作者：
Capua, Ilaria
中科院分区：
文献类型：
--
作者：
Bonfante, Francesco;Patrono, Livia Victoria;Capua, Ilaria
自1990年代中期以来，H9 N2亚型禽流感病毒在亚洲、远东和中东的家禽种群中流行。在家禽中建立的最广泛的谱系之一是G1谱系。自1997年首次发现以来，这一谱系经历了进一步的进化和重组，G1样H9 N2病毒仍在传播。在这项研究中，我们调查了鹌鹑和火鸡对H9 N2 G1谱系原型株（A/quail/Hong Kong/G1/97）的易感性。在这两种鸟类中进行了接触传播实验。用递增剂量的病毒（10（3）-10（6）EID 50/0.1 ml）经口-鼻感染动物，并在感染后4天（pi）在每个实验组中引入哨兵鸟。鹌鹑比火鸡更敏感，因为它们很容易在较低的攻击剂量下感染。有趣的是，火鸡的感染与更差的临床状况有关。在两个物种中均检测到传播。用小于或等于10（4）EID 50的剂量感染的鹌鹑将病毒传播给哨兵，而没有显示出任何疾病的迹象。这些发现强化了这样一种假设，即鹌鹑可能确保H9 N2病毒在家禽中永久存在，作为一个沉默的水库。(c)2013爱思唯尔有限公司版权所有。
Avian influenza viruses of the H9N2 subtype have circulated in the poultry population in Asia, Far and Middle East since the mid-1990s. One of the most widespread lineages established in poultry is the G1 lineage. This lineage has undergone further evolution and reassortment since its first detection in 1997 and G1-like H9N2 viruses still circulate. In this study we have investigated the susceptibility of quail and turkeys to the H9N2 G1-lineage prototype strain (A/quail/Hong Kong/G1/97). Contact transmission experiments were carried out in both avian species. Animals were infected oro-nasally with increasing doses of the virus (10(3)-10(6) EID50/0.1 ml) and sentinel birds were introduced 4 days post infection (pi) in each experimental group. Quail were more susceptible than turkeys, as they were readily infected with lower challenge doses. Interestingly, infection of turkeys was associated with worse clinical condition. Transmission was detected in both species. Quail infected with a dose less than or equal to 10(4) EID50 transmitted the virus to the sentinels without showing any signs of disease. These findings reinforce the hypothesis that quail may ensure the perpetuation of H9N2 viruses in poultry, acting as a silent reservoir. (c) 2013 Elsevier B.V. All rights reserved.