Antimicrobial and antioxidant activity of Garcinia brasiliensis extracts
Antimicrobial and antioxidant activity of Garcinia brasiliensis extracts
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DOI：
10.1016/j.sajb.2019.05.021
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发表时间：
2019-08-01
影响因子：
3.1
通讯作者：
Dias, D. F.
中科院分区：
文献类型：
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作者：
Naves, V. M. L.;dos Santos, M. H.;Dias, D. F.
本研究的目的是对巴西藤黄叶、皮和种子的乙醇提取物和水提物进行化学表征和生物活性评价。对提取物中的酚类化合物和类黄酮类化合物进行了定量，并进行了生物筛选，以验证所研究提取物的抗氧化和抑菌活性。提取物中酚类化合物含量最高的是叶水提物，含量为343.98+/-4.8mgGA/g，而黄酮类化合物在种子醇提物和叶水提物中含量最高，分别为47.10+/-0.2和45.62+/-0.7mgGA/g。种子乙醇提取物具有重要的抗氧化活性，对DPPH自由基的清除率为86.57%。另一方面，LAE具有很强的抗菌活性(对金黄色葡萄球菌的IC50为1微克/毫升)。采用毛细管电泳法进行高效液相分析，检测到葛根素-7‘-O-β-D-糖苷和葛根素的存在。对LAE的细胞毒作用进行了评估，在正常细胞中没有显示出细胞毒活性。考虑到这一点，结果表明，芦荟皂苷具有重要的抗菌和抗真菌活性，具有很大的抗菌潜力。(C)2019年萨博。爱思唯尔出版，版权所有。
The aim of this study was to chemically characterize and evaluate the biological activities of ethanolic and aqueous extracts from its leaf, bark and seed of Garcinia brasiliensis. The total content of phenolic compounds and flavonoids present in the extracts was quantified, and biological screenings were performed to verify antioxidant and antimicrobial activities of the studied extracts. The extract with the highest content of phenolic compounds was the leaf aqueous extract (LAE), presenting 343.98 +/- 4.8 mg GA/g. The most relevant flavonoid contents were observed in the seed ethanolic extract and in the leaf aqueous extract, presenting, respectively, 47.10 +/- 0.2 and 45.62 +/- 0.7 mg quercetin/g of extract. The important antioxidant activity was observed in the seed ethanolic extract, with 86.57% of DPPH free radical scavenging. On the other hand, the LAE displayed a great antimicrobial activity (IC50 of 1 mu g/mL against S. aureus). Analysis by high performance liquid chromatography was carried out using the LAE and the presence of fukugentin-7 ''-O-beta-D-glycoside (fukugiside) and fukugetin was detected. The cytotoxic effect of LAE was evaluated and showed no cytotoxic activity in normal cells. Considering this, the results show that the fukugiside present in LAE display an important antibacterial and antifungal activity, so this has great potential as antimicrobial agent. (C) 2019 SAAB. Published by Elsevier B.V. All rights reserved.