Cytopathological analysis of vitreous in intraocular lymphoma
Cytopathological analysis of vitreous in intraocular lymphoma
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DOI：
10.1038/sj.eye.6702965
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发表时间：
2008-02-01
期刊：
影响因子：
3.9
通讯作者：
Pawade, J.
中科院分区：
文献类型：
--
作者：
Intzedy, L.;Teoh, S. C. B.;Pawade, J.
目的探讨玻璃体标本细胞病理学分析在原发性眼内淋巴瘤（PIOL）诊断中的应用。研究对象1999 ~ 2006年收治的7例难治性后葡萄膜炎患者均行PIOL术。方法描述葡萄膜炎的临床特征及细胞病理学标本。所有患者均接受玻璃体切除术，并将样本置于正式盐水中或新鲜制备。石蜡包埋后产生的细胞块，免疫染色，聚合酶链反应performed.Results五名妇女（71.4%）和两名男子（28.6%）（平均年龄67.7岁）。5例患者在就诊后6个月内进行了诊断性玻璃体切除术，但2例患者的诊断延迟了2年。葡萄膜炎是双边的两名患者。玻璃体标本的细胞学和免疫组化染色显示PIOL在所有患者中，和PCR显示单带免疫球蛋白重链重排的6个tested.Conclusions PIOL的诊断是困难的，由于小体积的样本，恶性细胞数量少，标本制备不充分。我们的方法与新鲜样本的分析，免疫组化和PCR分析表明，诊断率高，减少诊断延迟。
Objective To describe the cytopathological method used in the analysis of vitreous samples in the diagnosis of primary intraocular lymphoma (PIOL). Participants Seven patients with refractory posterior uveitis referred to a regional ocular inflammatory service were diagnosed as having PIOL between 1999 and 2006.Methods Clinical features of the uveitis and cytopathological preparation of the samples were described. All patients underwent vitrectomy and samples were placed in formal saline or prepared fresh. Following paraffin embedding generating a cell block, immunostaining, and polymerase chain reactions were performed.Results Five women (71.4%) and two men (28.6%) (mean age 67.7 years) were included. Five patients had diagnostic vitrectomy performed within 6 months of presentation, but in two patients diagnosis was delayed up to 2 years. Uveitis was bilateral in two patients. Cytologic and immunohistochemical staining prepared from the vitreous specimens showed PIOL in all patients, and PCR displayed single band of immunoglobulin heavy chain rearrangement in five out of six samples tested.Conclusions Diagnosis of PIOL is difficult due to small volume of sample with low number of malignant cells and inadequate preparation of samples. Our method of analysis with fresh samples together with immunohistochemistry and PCR analysis demonstrates a high yield of diagnosis reducing diagnostic delay.