IMMUNOMODULATORY EFFECTS OF 3 MACROLIDES, MIDECAMYCIN ACETATE, JOSAMYCIN, AND CLARITHROMYCIN, ON HUMAN T-LYMPHOCYTE FUNCTION IN-VITRO
IMMUNOMODULATORY EFFECTS OF 3 MACROLIDES, MIDECAMYCIN ACETATE, JOSAMYCIN, AND CLARITHROMYCIN, ON HUMAN T-LYMPHOCYTE FUNCTION IN-VITRO
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DOI：
10.1128/aac.38.11.2643
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发表时间：
1994-11-01
影响因子：
4.9
通讯作者：
IWAMOTO, K
中科院分区：
文献类型：
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作者：
MORIKAWA, K;OSEKO, F;IWAMOTO, K
在体外研究了三种大环内酯类抗生素，即醋酸麦迪霉素、交沙霉素和克拉霉素对人T细胞功能的影响。醋酸麦迪霉素和交沙霉素在1.6和8 μ g/ml之间的浓度下抑制多克隆T细胞有丝分裂原刺激的外周血单核细胞的增殖反应。在较高浓度（40至200 μ g/ml）下，所有这些药物均显示出显著的抑制作用。在1.6至40 μ g/ml的浓度下，这些药物以剂量依赖性方式抑制由促分裂原刺激的T细胞诱导的白细胞介素-2（IL-2）的产生，但不抑制IL-2受体（CD 25）的表达。因此，对T淋巴细胞增殖的抑制作用似乎是基于这些药物抑制T细胞产生IL-2的能力。在相同浓度下，药物也抑制混合淋巴细胞反应。用这些大环内酯类和已知的免疫抑制剂如FK 506和环孢菌素A联合治疗导致：增加对T细胞增殖的抑制。抗生素的免疫调节特性可能与移植患者和炎性疾病患者的免疫应答调节具有临床相关性。
The effect of three macrolide antibiotics, midecamycin acetate, josamycin, and clarithromycin, on human T-cell function was investigated in vitro. Midecamycin acetate and josamycin suppressed the proliferative response of peripheral blood mononuclear cells stimulated by polyclonal T-cell mitogens at concentrations between 1.6 and 8 mu g/ml. At higher concentrations (40 to 200 mu g/ml), all these drugs showed a marked inhibitory effect. At concentrations of 1.6 to 40 mu g/ml, these drugs suppressed interleukin-2 (IL-2) production induced by mitogen-stimulated T cells, but not the expression of IL-2 receptor (CD25), in a dose-dependent manner. Therefore, the suppressive action on T-lymphocyte proliferation seems to be based on the ability of these drugs to inhibit IL-2 production by T cells. The drugs also inhibited mixed lymphocyte reaction at the same concentrations. Combined treatment with these macrolides and the known immunosuppressants such as FK506 and cyclosporin A resulted in an:increased inhibition of T-cell proliferation. The immunomodulatory properties of the antibiotics may have clinical relevance for modulation of the immune response in transplant patients and in patients with inflammatory diseases.