Analysis of the traditional medicine YiGan San by the fragmentation patterns of cadambine indole alkaloids using HPLC coupled with high-resolution MS
Analysis of the traditional medicine YiGan San by the fragmentation patterns of cadambine indole alkaloids using HPLC coupled with high-resolution MS
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DOI：
10.1002/jssc.201300804
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发表时间：
2013-12-01
影响因子：
3.1
通讯作者：
Wang, SuiLou
中科院分区：
文献类型：
--
作者：
Gai, YaNan;Chen, Han;Wang, SuiLou
一肝散 (YGS) 长期以来一直用于日本和中国的传统民间医学，是治疗阿尔茨海默病的有效且新颖的治疗剂。在本研究中，设计了一种基于 HPLC 结合二极管阵列检测和四极杆 TOF MS (Q-TOF-MS) 的快速、灵敏的方法来揭示 YGS 的化学成分。 YGS 鉴定并归属了 36 种化合物，其中包括 14 种生物碱、9 种β-内酯、6 种黄酮类化合物、3 种三萜皂苷类、2 种小分子有机酸和 2 种其他类型的化合物。此外，首次总结了钩藤中某亚型吲哚生物碱的准确片段重量和MS/MS裂解反应，实现了无对照品的快速鉴定。首次采用 HPLC 结合三重四极杆 MS 方法对 11 种主要成分进行全面定量。使用三段开关来实现这种多组分识别。两种来源的 YGS 中柴胡皂苷 B-2 和异甘草苷的含量差异极大，高达 700 倍，而柴胡皂苷 B-2 和异甘草苷具有抗炎和抗抑郁作用。所开发的定性和定量方法被证明是精确、准确和可重复的。
YiGan San (YGS) has long been used in traditional Japanese and Chinese folk medicine and serves as a potent and novel therapeutic agent to treat Alzheimer's disease. In the present study, a rapid and sensitive method based on HPLC coupled with diode-array detection and quadrupole TOF MS (Q-TOF-MS) was designed to reveal the chemical constituents of YGS. Thirty-six compounds were identified and assigned in YGS, including 14 alkaloids, nine -lactones, six flavonoids, three triterpenoid saponinares, two small molecular organic acids, and two other types of compounds. In addition, the accurate fragment weight and MS/MS fragmentation reactions of a subtype indole alkaloid in Uncariae ramulus cum uncis were summarized for the first time to realize rapid identification without reference substances. For the first time, 11 major constituents were comprehensively quantified with a HPLC coupled with triple-quadrupole MS method. A three-section switch was used to realize such multicomponent identification. The contents of saikosaponin B-2 and isoliquiritin, which produce anti-inflammatory and antidepressant-like effects, were extremely different, up to 700 times, in two sources of YGS. The developed qualitative and quantitative method was proved to be precise, accurate, and reproducible.