Photoactivatable fluorophores for durable labelling of individual cells
Photoactivatable fluorophores for durable labelling of individual cells
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可光激活的荧光团用于对单个细胞进行持久标记
DOI：
10.1039/d1cc01488a
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发表时间：
2021
影响因子：
4.9
通讯作者：
Urano Yasuteru
中科院分区：
文献类型：
--
作者：
Kashima Hiroki;Kamiya Mako;Obata Fumiaki;Kojima Ryosuke;Nakano Shotaro;Miura Masayuki;Urano Yasuteru
我们已经设计并开发了非荧光的、细胞可渗透的可光活化的荧光团，可光活化的SPiDER（paSPiDER），其在光照射时表现出荧光活化，伴随着生成与细胞内蛋白共价结合的醌甲基化物中间体。荧光信号可持续24小时，耐固定，与免疫染色相容，并且可以在单细胞分辨率下实现选择性细胞标记。
We have designed and developed non-fluorescent, cell-permeable photoactivatable fluorophores, photoactivatable SPiDERs (paSPiDERs), which exhibit fluorescence activation upon light irradiation, accompanied by the generation of a quinone methide intermediate that binds covalently to intracellular proteins. The fluorescence signal is durable for 24 hours, resistant to fixation and compatible with immunostaining, and selective cell labeling can be achieved at single-cell resolution.