Effects of cell adhesion motif, fiber stiffness, and cyclic strain on tenocyte gene expression in a tendon mimetic fiber composite hydrogel.
Effects of cell adhesion motif, fiber stiffness, and cyclic strain on tenocyte gene expression in a tendon mimetic fiber composite hydrogel.
复制标题
细胞粘附基序、纤维刚度和循环应变对仿腱纤维复合水凝胶中肌腱细胞基因表达的影响。
DOI：
10.1016/j.bbrc.2018.03.203
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发表时间：
2018
影响因子：
3.1
通讯作者：
Bryant,StephanieJ
中科院分区：
文献类型：
--
作者：
Patel,Dharmesh;Sharma,Sadhana;Screen,HazelRC;Bryant,StephanieJ
We recently developed a fiber composite consisting of tenocytes seeded onto discontinuous fibers embedded within a hydrogel, designed to mimic physiological tendon micromechanics of tension and shear. This study examined if cell adhesion peptide (DGEA or YRGDS), fiber modulus (50 or 1300 kPa) and/or cyclic strain (5% strain, 1 Hz) influenced bovine tenocyte gene expression. Ten genes were analyzed and none were sensitive to peptide or fiber modulus in the absence of cyclic tensile strain. Genes associated with tendon (SCXandTNMD), collagens (COL1A1,COL3A1,COL11A1), and matrix remodelling (MMP1,MMP2, andTIMP3) were insensitive to cyclic strain. Contrarily, cyclic strain up-regulatedIL6by 30-fold andMMP3by 10-fold in soft YRGDS fibers.IL6expression in soft YRGDS fibers was 5.7 and 3.3-fold greater than in soft DGEA fibers and stiff RGD fibers, respectively, under cyclic strain. Our findings suggest that changes in the surrounding matrix can influence catabolic genes in tenocytes when cultured in a complex strain environment mimicking that of tendon, while having minimal effects on tendon and homeostatic genes.
