Tissue inhibitor of metalloproteinase gene from pearl oyster Pinctada martensii participates in nacre formation
Tissue inhibitor of metalloproteinase gene from pearl oyster Pinctada martensii participates in nacre formation
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马氏珠母贝金属蛋白酶基因组织抑制剂参与珍珠层形成
DOI：
10.1016/j.bbrc.2014.05.118
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发表时间：
2014
影响因子：
3.1
通讯作者：
Chen Weiyao
中科院分区：
文献类型：
--
作者：
Yan Fang;Jiao Yu;Deng Yuewen;Du Xiaodong;Huang Ronglian;Wang Qingheng;Chen Weiyao
组织金属蛋白酶抑制剂（Tissue inhibitors of metalloproteinases, TIMPs）是基质金属蛋白酶的天然抑制剂，在调节细胞外基质转换、组织重塑和骨形成中发挥重要作用。本研究描述了马氏珠贝（pinctada martensii）中TIMP的分子特征及其在珍珠形成中的潜在功能。TIMP基因的cDNA。马氏体（Pm-TIMP）全长901 bp，包含51 bp的5′未翻译区（UTR）、169 bp的3′未翻译区（UTR）和681 bp的开放阅读片段（ORF），编码226个氨基酸，估计分子量为23.37 kDa，理论等电点为5.42；预测的氨基酸序列具有1个信号肽、13个半胱氨酸残基、1个n端结构域和1个c端结构域，与其他物种相似。氨基酸多重比对表明，Pm-TIMP与长牡蛎的同源性最高（41%）。组织表达分析表明，Pm-TIMP在珠质形成相关组织中高表达，包括珠膜和珠囊。通过RNA干扰（RNAi）技术降低白衣中Pm-TIMP基因的表达后，壳的内珍珠层出现生长紊乱。这些结果表明，本研究获得的Pm-TIMP参与了珍珠质的形成。
Tissue inhibitors of metalloproteinases (TIMPs) are nature inhibitors of matrix metalloproteinases and play a vital role in the regulation of extracellular matrix turnover, tissue remodeling and bone formation. In this study, the molecular characterization of TIMP and its potential function in nacre formation was described in pearl oysterPinctada martensii. The cDNA of TIMP gene inP. martensii(Pm-TIMP) was 901 bp long, containing a 5′ untranslated region (UTR) of 51 bp, a 3′ UTR of 169 bp, and an open reading fragment (ORF) of 681 bp encoding 226 amino acids with an estimated molecular mass of 23.37 kDa and a theoretical isoelectric point of 5.42; The predicted amino acid sequence had a signal peptide, 13 cysteine residues, a N-terminal domain and a C-terminal domain, similar to that from other species. Amino acid multiple alignment showed Pm-TIMP had the highest (41%) identity to that fromCrassostrea gigas. Tissue expression analysis indicated Pm-TIMP was highly expressed in nacre formation related-tissues, including mantle and pearl sac. After decreasing Pm-TIMP gene expression by RNA interference (RNAi) technology in the mantle pallium, the inner nacreous layer of the shells showed a disordered growth. These results indicated that the obtained Pm-TIMP in this study participated in nacre formation.