Effect of dietary fatty acids on expression of lipogenic enzymes and fatty acid profile in tissues of bulls
Effect of dietary fatty acids on expression of lipogenic enzymes and fatty acid profile in tissues of bulls
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DOI：
10.1017/s1751731110000431
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发表时间：
2010-05-01
期刊：
影响因子：
3.6
通讯作者：
Doran, O.
中科院分区：
文献类型：
--
作者：
Herdmann, A.;Nuernberg, K.;Doran, O.
本研究调查了日粮亚麻酸 (C18:3n-3) 与亚油酸 (C18:2n-6) 对公牛最长肌和皮下脂肪组织中关键脂肪生成酶、乙酰辅酶 A 羧化酶 (ACC)、硬脂酰辅酶 A 去饱和酶 (SCD) 和 Delta 6 去饱和酶 (Delta 6d) 的脂肪酸组成和蛋白质表达的影响。饮食中补充 C18:3n-3 伴随着肌肉中 n-3 脂肪酸水平的增加，导致 n-6/n-3 比率下降。富含n-3多不饱和脂肪酸（PUFA）的饮食显着抑制肌肉和皮下脂肪组织中SCD蛋白的表达，并减少肌肉中Delta 6d的表达。饮食对 ACC 蛋白表达没有显着影响。 Delta 6d 表达的抑制与肌肉中 n-6 PUFA 水平的降低相关，而 SCD 蛋白的抑制与脂肪组织中较低的油酸 (C18:1 cis-9) 含量相关。与最长肌相比，皮下脂肪组织中 ACC、SCD 和 Delta 6d 蛋白的表达相对较高。有人提出，反刍动物中脂肪酸组成的饮食控制至少部分是通过脂肪生成酶表达的调节来介导的，并且牛脂肪生成酶表达的调节是组织特异性的。
This study investigated the effects of dietary linolenic acid (C18:3n-3) v. linoleic acid (C18:2n-6) on fatty acid composition and protein expression of key lipogenic enzymes, acetyl-CoA carboxylase (ACC), stearoyl-CoA desaturase (SCD) and delta 6 desaturase (Delta 6d) in longissimus muscle and subcutaneous adipose tissue of bulls. Supplementation of the diet with C18:3n-3 was accompanied by an increased level of n-3 fatty acids in muscle which resulted in decrease of n-6/n-3 ratio. The diet enriched with n-3 polyunsaturated fatty acids (PUFAs) significantly inhibited SCD protein expression in muscle and subcutaneous adipose tissue, and reduced the Delta 6d expression in muscle. There was no significant effect of the diet on ACC protein expression. Inhibition of the Delta 6d expression was associated with a decrease in n-6 PUFA level in muscles, whereas repression of SCD protein was related to a lower oleic acid (C18:1 cis-9) content in the adipose tissue. Expression of ACC, SCD and Delta 6d proteins was found to be relatively higher in subcutaneous adipose tissue when compared with longissimus muscle. It is suggested that dietary manipulation of fatty acid composition in ruminants is mediated, at least partially, through the regulation of lipogenic enzymes expression and that regulation of the bovine lipogenic enzymes expression is tissue specific.