Intratumoral FOXP3+Regulatory T Cells in Diffuse Large B-Cell Lymphoma
Intratumoral FOXP3+Regulatory T Cells in Diffuse Large B-Cell Lymphoma
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DOI：
10.1097/pai.0000000000000335
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发表时间：
2017-09-01
影响因子：
1.6
通讯作者：
Kora, Mona Abd El-Hamid M.
中科院分区：
文献类型：
--
作者：
El-Dien, Marwa M. Serag;Abdou, Asmaa G.;Kora, Mona Abd El-Hamid M.
弥漫性大B细胞淋巴瘤（DLBCL）是埃及和全世界最常见的非霍奇金淋巴瘤亚型。基因表达谱将DLBCL分类为：生发中心B细胞样（GCB）和非生发中心B细胞样（非GCB）DLBCL。Hans算法与基因表达谱分析结果具有较高的一致性。调节性T细胞（Regulatory T cells，Tcells）是淋巴瘤细胞与宿主微环境相互作用的重要调节因子。FOXP3是一个流行的单一标记的甲状腺肿。关于TdR在高级别淋巴瘤（如DLBCL）中可能发挥的作用的信息很少。本研究的目的是评估FOXP3+ T细胞在DLBCL中的预后影响。该研究对70例存档病例（61例新发DLBCL和9例反应性滤泡增生病例）进行。根据Hans算法将DLBCL分为GCB组和非GCB组。所有研究病例均进行FOXP3免疫染色。反应性病例中FOXP3+ T细胞密度高于DLBCL（P = 0.000）。在DLBCL病例中，FOXP3表达与游离脾（P = 0.02）、早期（P = 0.05）、中心母细胞变异（P = 0.003）和无坏死（P = 0.05）相关。在老年病例中，PS良好（P = 0.02）、修订的国际预后指数非常好和良好（P = 0.002）以及年龄校正的国际预后指数> 60（P = 0.01）的低风险病例中FOXP3的密度显著较高。FOXP3阴性的非老年DLBCL病例与脾脏受累显著相关（P = 0.005）。FOXP3高表达的DLBCL患者生存期较长（P = 0.03）。DLBCL背景中的T细胞可能在调节肿瘤进展中起作用。它们的存在与DLBCL的有利预后参数相关。
Diffuse large B-cell lymphoma (DLBCL) is the most common subtype of non-Hodgkin lymphoma in Egypt and worldwide. Gene expression profiling classifies DLBCL into: germinal center B cell-like (GCB) and non germinal center B cell-like (non-GCB) DLBCL. Hans' algorithm has high concordance with gene expression profiling results. Regulatory T cells (Tregs) represent important modulators for the interaction between lymphoma cells and host microenvironment. FOXP3 is a popular single marker for Tregs. There is little information about the possible role of Tregs in high-grade lymphoma such as DLBCL. This study aims to assess the prognostic impact of FOXP3+ Tregs in DLBCL. The study was carried out on 70 archival cases (61 de novo DLBCL and 9 reactive follicular hyperplasia cases). DLBCL cases were classified into GCB and non-GCB groups using Hans' algorithm. All studied cases are subjected to FOXP3 immunostaining. Density of FOXP3+ Tregs was higher in reactive cases compared with DLBCL (P = 0.000). In DLBCL cases, FOXP3 expression was associated with free spleen (P = 0.02), early stage (P = 0.05), centroblastic variant (P = 0.003), and absence of necrosis (P = 0.05). In germinal cases, density of FOXP3 was significantly higher in cases with good PS (P = 0.02), very good and good revised international prognostic index (P = 0.002), and low-risk age-adjusted international prognostic index > 60 (P = 0.01). Non germinal DLBCL cases with negative FOXP3 were significantly associated with splenic involvement (P = 0.005). DLBCL cases with high FOXP3 have longer survival (P = 0.03). T cells in the background of DLBCL may play a role in modulation of tumor progression. Their presence is associated with favorable prognostic parameters in DLBCL.