Simultaneous Quantification of Nucleosides and Nucleotides from Biological Samples
Simultaneous Quantification of Nucleosides and Nucleotides from Biological Samples
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DOI：
10.1007/s13361-019-02140-7
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发表时间：
2019-06-01
影响因子：
3.2
通讯作者：
Zhang, Xiang
中科院分区：
文献类型：
--
作者：
He, Liqing;Wei, Xiaoli;Zhang, Xiang
我们报告了一种同时定量生物样品中核苷和核苷酸的反相色谱质谱（LC-MS）方法，其中化合物鉴定通过分层方法实现，化合物定量通过外部校准实现。平台开发共使用了65个核苷和核苷酸的正品标准。化合物的检出限为0.05 ~ 1.25mol/L，定量限为0.10 ~ 2.50mol/L。采用所建立的方法，对人血浆、人尿和大鼠肝脏中的核苷和核苷酸进行了定量。从人尿中鉴定出79种核苷和核苷酸，其中28种的定量浓度为13.0nmol/L-151mol/L。从人血浆中鉴定出55种核苷和核苷酸，其中22种的定量浓度为1.21nmol/L-8.54mol/L。从大鼠肝脏中鉴定出51种核苷和核苷酸，其中23种的定量浓度为1.03nmol/L-31.7mol/L。这些结果表明，该方法可用于研究生物样品中核苷和核苷酸的浓度变化，以发现生物标志物或阐明疾病机制。
We report a reverse phase chromatography mass spectrometry (LC-MS) method for simultaneous quantification of nucleosides and nucleotides from biological samples, where compound identification was achieved by a tier-wise approach and compound quantification was achieved via external calibration. A total of 65 authentic standards of nucleosides and nucleotides were used for the platform development. The limit of detection (LOD) of those compounds ranged from 0.05nmol/L to 1.25mol/L, and their limit of quantification (LOQ) ranged from 0.10nmol/L to 2.50mol/L. Using the developed method, nucleosides and nucleotides from human plasma, human urine, and rat liver were quantified. Seventy-nine nucleosides and nucleotides were identified from human urine and 28 of them were quantified with concentrations of 13.0nmol/L-151mol/L. Fifty-five nucleosides and nucleotides were identified from human plasma and 22 of them were quantified with concentrations of 1.21nmol/L-8.54mol/L. Fifty-one nucleosides and nucleotides were identified from rat liver and 23 were quantified with concentrations of 1.03nmol/L-31.7mol/L. These results demonstrate that the developed method can be used to investigate the concentration change of nucleosides and nucleotides in biological samples for the purposes of biomarker discovery or elucidation of disease mechanisms.