Mass Spectrometric Imaging Using Laser Ablation and Solvent Capture by Aspiration (LASCA)
Mass Spectrometric Imaging Using Laser Ablation and Solvent Capture by Aspiration (LASCA)
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DOI：
10.1007/s13361-015-1176-0
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发表时间：
2015-09-01
影响因子：
3.2
通讯作者：
Sunner, Jan
中科院分区：
文献类型：
--
作者：
Brauer, Jonathan I.;Beech, Iwona B.;Sunner, Jan
一种新的接口环境，激光烧蚀为基础的质谱成像（MSI）被称为激光烧蚀和溶剂捕获抽吸（LASCA），其性能证明使用选定的，未改变的生物材料。LASCA采用脉冲2.94 μ m激光束进行试样烧蚀。激光羽流中的烧蚀材料被收集在具有电场增强捕获的悬挂溶剂液滴上，然后将液滴和粗气溶胶形式的剩余羽流材料吸入收集毛细管中。随后在10 μ L体积的分液漏斗中分离气相和液相，并在高质量分辨率Q-ToF质谱仪中通过电喷雾电离分析溶液。LASCA系统将MSI中的采样和电离步骤分开，并将激光羽流采样和电喷雾电离（ESI）的高效率与高质量分辨率MS相结合。从单个消融点（像素）检测到多达2000种不同的化合物。使用LASCA平台，快速（每像素6秒），高灵敏度，高质量分辨率的“接收”生物材料的环境成像是常规和可再现的。
A novel interface for ambient, laser ablation-based mass spectrometric imaging (MSI) referred to as laser ablation and solvent capture by aspiration (LASCA) is presented and its performance demonstrated using selected, unaltered biological materials. LASCA employs a pulsed 2.94 mu m laser beam for specimen ablation. Ablated materials in the laser plumes are collected on a hanging solvent droplet with electric field-enhanced trapping, followed by aspiration of droplets and remaining plume material in the form of a coarse aerosol into a collection capillary. The gas and liquid phases are subsequently separated in a 10 mu L-volume separatory funnel, and the solution is analyzed with electrospray ionization in a high mass resolution Q-ToF mass spectrometer. The LASCA system separates the sampling and ionization steps in MSI and combines high efficiencies of laser plume sampling and of electrospray ionization (ESI) with high mass resolution MS. Up to 2000 different compounds are detected from a single ablation spot (pixel). Using the LASCA platform, rapid (6 s per pixel), high sensitivity, high mass-resolution ambient imaging of "as-received" biological material is achieved routinely and reproducibly.