Novel technologies in cfDNA analysis and potential utility in clinic.
Novel technologies in cfDNA analysis and potential utility in clinic.
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DOI：
10.21147/j.issn.1000-9604.2021.06.07
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发表时间：
2021-12-31
期刊：
影响因子：
--
通讯作者：
Lan X
中科院分区：
文献类型：
--
作者：
Li J;Xu M;Peng J;Wang J;Zhao Y;Wu W;Lan X
血浆游离DNA（cfDNA）的谱分析正迅速成为肿瘤诊断、监测和预后的有价值的工具。多种血浆cfDNA技术已被常规或新兴使用，包括突变、拷贝数改变、基因融合和DNA甲基化的分析。最近，cfDNA分析的新技术已经在实验室中开发出来，并可能反映肿瘤组织中表观遗传修饰，免疫微环境和微生物组的状态。在这篇综述中，作者讨论了目前cfDNA检测的原理、方法和效果，并概述了可能在不久的将来为临床应用提供信息的研究。
The profiling of plasma cell-free DNA (cfDNA) is becoming a valuable tool rapidly for tumor diagnosis, monitoring and prognosis. Diverse plasma cfDNA technologies have been in routine or emerging use, including analyses of mutations, copy number alterations, gene fusions and DNA methylation. Recently, new technologies in cfDNA analysis have been developed in laboratories, and potentially reflect the status of epigenetic modification, the immune microenvironment and the microbiome in tumor tissues. In this review, the authors discuss the principles, methods and effects of the current cfDNA assays and provide an overview of studies that may inform clinical applications in the near future.