Expression of PBMC apoptosis-related factors in patients with chronic hepatitis B and their relationships with clinical prognosis.
Expression of PBMC apoptosis-related factors in patients with chronic hepatitis B and their relationships with clinical prognosis.
复制标题
DOI：
10.3892/etm.2017.5299
复制
发表时间：
2017-12
影响因子：
2.7
通讯作者：
Liang Y
中科院分区：
文献类型：
--
作者：
Guo L;Liang Y
本研究探讨慢性B型肝炎患者外周血单个核细胞（PBMC）中促肝细胞凋亡相关因子的表达及其与临床预后的关系。选择2015年3月至2016年2月在徐州市人民医院住院诊断为慢性B型肝炎的患者62例为观察组，选择在体检中心体检的健康体检者60例为对照组。收集患者PBMC，采用实时荧光定量PCR检测凋亡分子FAS、CASP 3、CASP 8和CASP 9的mRNA表达水平。采用酶联免疫吸附试验（ELISA）检测血浆中乙肝相关基因蛋白表达水平，采用实时荧光定量PCR法检测血清HBV-DNA。观察组FAS、CASP 3、CASP 8和CASP 9的mRNA和蛋白表达水平均显著高于对照组（P<0. 05）。观察组HBV DNA拷贝数的阳性率和对数值均显著高于对照组（P<0.05）。Pearson相关分析显示FAS、CASP 3、CASP 8、CASP 9与HBVDNA呈正相关（P<0.05）。HBV-DNA阴性组FAS、CASP 3、CASP 8、CASP 9 mRNA表达水平均显著低于HBV-DNA阳性组（P<0.05）。PBMCs凋亡在慢性B型肝炎的发生、发展中起重要作用，并与血清病毒复制水平及预后密切相关。
The present study was conducted to investigate the expression of apoptosis-related factors in peripheral blood mononuclear cells (PBMC) of patients with chronic hepatitis B and their correlation to clinical prognosis. Sixty-two patients that were diagnosed with chronic hepatitis B were admitted to Xuzhou Hospital from March 2015 to February 2016 and were enrolled as the observation group, while 60 healthy subjects who were examined in the health examination center were selected as the control group. The PBMC of patients were collected, and mRNA expression levels of the apoptotic molecules (FAS, CASP3, CASP8 and CASP9) were measured using real-time fluorescence quantitative PCR. The protein expression levels of apoptosis-related genes in the plasma were detected using enzyme-linked immunosorbent assay (ELISA) and the serum HBV-DNA was quantitatively measured using real-time fluorescence quantitative PCR. The mRNA and protein expression levels of FAS, CASP3, CASP8 and CASP9 in the observation group were significantly higher than those in the control group (P<0.05). The positive rate and log value of the copy amount of HBV DNA in the observation group were significantly higher than those in the control group (P<0.05). The Pearson correlation coefficient analysis showed that FAS, CASP3, CASP8 and CASP9 were positively correlated with HBV DNA (P<0.05). The mRNA expression levels of FAS, CASP3, CASP8 and CASP9 in patients with negative HBV-DNA were significantly lower than those with positive HBV-DNA (P<0.05). Apoptosis of PBMCs play an important role in the occurrence and development of chronic hepatitis B, and is closely correlated to the level of serum virus replication and prognosis.
登录
查看更多内容
影响因子：
3.2
作者：
Peng, Cheng-Yuan;Hsieh, Tsung-Cheng;Kao, Jia-Horng
通讯作者：
Kao, Jia-Horng
影响因子：
2.9
作者：
Martins, Francisco d'Oliveira;Gomes, Bruno Costa;Rueff, Jose
通讯作者：
Rueff, Jose
影响因子：
12.4
作者：
Hong CA;Nam YS
通讯作者：
Nam YS
影响因子：
13.5
作者：
Roberts, Henry;Kruszon-Moran, Deanna;Holmberg, Scott D.
通讯作者：
Holmberg, Scott D.
影响因子：
4
作者：
Lv, Junhong;Lin, Shaohuan;Li, Qiling
通讯作者：
Li, Qiling