Exploration of candidate biomarkers for human psoriasis based on gas chromatography-mass spectrometry serum metabolomics
Exploration of candidate biomarkers for human psoriasis based on gas chromatography-mass spectrometry serum metabolomics
复制标题
基于气相色谱-质谱血清代谢组学的人类银屑病候选生物标志物探索
DOI：
10.1111/bjd.15008
复制
发表时间：
2017-03-01
影响因子：
10.3
通讯作者：
Yu, Y.
中科院分区：
文献类型：
--
作者：
Kang, H.;Li, X.;Yu, Y.
研究背景近年来的研究表明代谢途径失调与银屑病发病机制有关。然而，广泛的，公正的代谢分析银屑病患者尚未完全探讨。代谢组是蛋白质组学或细胞过程的终产物，可能与银屑病的发病机制密切相关。目的确定银屑病患者和健康对照者血清代谢组学谱的差异，以确定银屑病患者潜在的生物标志物。（14名银屑病患者和15名性别和年龄匹配的健康对照）。采用气相色谱-质谱联用技术，结合全扫描和选择离子监测模式，对银屑病患者和健康人的血清代谢物进行分析。与健康人相比，银屑病患者体内天冬酰胺、天冬氨酸、异亮氨酸、苯丙氨酸、鸟氨酸、脯氨酸等氨基酸水平升高，乳酸、尿素水平升高，巴豆酸、壬二酸、乙醇胺、胆固醇水平降低。这些代谢紊乱可能源于对蛋白质生物合成和角质形成细胞过度增殖的需求增加。我们的研究结果可能有助于阐明银屑病的发病机制，并为早期诊断和治疗干预提供见解。
Background Recent studies have shown that dysregulated metabolic pathways are linked to psoriasis pathogenesis. However, an extensive, unbiased metabolic analysis in patients with psoriasis has not been completely explored. The metabolome represents the end products of proteomics or cellular processes that may be closely associated with the pathogenesis of psoriasis.Objectives To determine the differences in serum metabolomic profiles among patients with psoriasis and healthy controls with the goal of identifying potential biomarkers in patients with psoriasis.Materials and methods Serum metabolomic profiles from 29 subjects (14 patients with psoriasis and 15 sex-and age-matched healthy controls). The serum metabolites were analysed by gas chromatography-mass spectrometry based on a combined full scan and selected-ion monitoring mode.Results Multivariate statistical analysis of metabolomics data revealed altered serum metabolites between the patients with psoriasis and healthy individuals. Compared with healthy individuals, patients with psoriasis had higher levels of amino acids including asparagine, aspartic acid, isoleucine, phenylalanine, ornithine and proline; higher levels of lactic acid and urea; and lower levels of crotonic acid, azelaic acid, ethanolamine and cholesterol.Conclusions It appears that the glycolysis pathway and amino acid metabolic activity are increased in patients with psoriasis. These metabolic perturbations may stem from increased demand for protein biosynthesis and keratinocyte hyperpro-liferation. Our findings may help to elucidate the pathogenesis of psoriasis and provide insights into early diagnosis and therapeutic intervention.