Frizzled receptors signal through G proteins.
Frizzled receptors signal through G proteins.
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DOI：
10.1016/j.cellsig.2013.03.009
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发表时间：
2013-06
影响因子：
4.8
通讯作者：
Baranski TJ
中科院分区：
文献类型：
--
作者：
Nichols AS;Floyd DH;Bruinsma SP;Narzinski K;Baranski TJ
Frizzled receptors have long been thought to couple to G proteins but biochemical evidence supporting such an interaction has been lacking. Here we expressed mammalian Wnt-Frizzled fusion proteins in Saccharomyces cerevisiae and tested the receptors’ ability to activate the yeast mitogen-activated protein kinase (MAPK) pathway via heterotrimeric G proteins. Our results show that Frizzled receptors can interact with Gαi, Gαq, and Gαs proteins, thus confirming that Frizzled functions as a G protein coupled receptor (GPCR). However, the activity level of Frizzled-mediated G protein signaling was much lower than that of a typical GPCR and, surprisingly, was highest when coupled to Gαs. The Frizzled/Gαs interaction was further established in vivo as Drosophila expressing a loss-of-function Gαs allele rescued the photoreceptor differentiation phenotype of Frizzled mutant flies. Together, these data point to an important role for Frizzled as a nontraditional GPCR that preferentially couples to Gαs heterotrimeric G proteins.
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影响因子：
56.9
作者：
Behrens, J;Jerchow, BA;Birchmeier, W
通讯作者：
Birchmeier, W
DOI：
10.1073/pnas.96.7.3546
发表时间：
1999-03-30
影响因子：
11.1
作者：
Hsieh, JC;Rattner, A;Nathans, J
通讯作者：
Nathans, J
DOI：
10.1073/pnas.96.25.14383
发表时间：
1999-12-07
影响因子：
11.1
作者：
Liu, XX;Liu, T;Wang, HY
通讯作者：
Wang, HY
影响因子：
11.4
作者：
Olesnicky, NS;Brown, AJ;Casselton, LA
通讯作者：
Casselton, LA
影响因子：
4.8
作者：
Ma, Li;Wang, Hsien-yu
通讯作者：
Wang, Hsien-yu