Molecular identification and structural detection of anti-cancer compound from marine Streptomyces akiyoshiensis GRG 6 (KY457710) against MCF-7 breast cancer cells
Molecular identification and structural detection of anti-cancer compound from marine Streptomyces akiyoshiensis GRG 6 (KY457710) against MCF-7 breast cancer cells
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海洋秋吉链霉菌 GRG 6 (KY457710) 抗 MCF-7 乳腺癌细胞抗癌化合物的分子鉴定和结构检测
DOI：
10.1016/j.jksus.2020.10.008
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发表时间：
2020-12-01
影响因子：
3.8
通讯作者：
Li, Wen-Jun
中科院分区：
文献类型：
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作者：
Rajivgandhi, Govindan Nadar;Ramachandran, Govindan;Li, Wen-Jun
本研究旨在检测海洋链霉菌（Streptomyces akiyoshiensis）GRG 6（KY 457710）对MCF-7乳腺癌细胞的抗癌活性。薄层层析部分纯化后，移动了三个不同的活性点。在这些斑点中，提取物的第三斑点显示出对MCF-7人乳腺癌细胞的优异抗癌特性。通过GC-MS数据解释证实了部分纯化提取物中存在的可用抗癌化合物。通过制备型HPLC纯化化合物的三个级分，并通过分析型HPLC确认它们各自的光谱。抗癌活性组分经制备型HPLC分离纯化后，在100 mg/mL浓度下进行细胞毒性试验。在用GC-MS数据解释后，通过LC-MS分析检测到抗癌化合物吡咯并[1，2-a]吡嗪-1，4-二酮，六氢-3。利用相差显微镜和荧光显微镜技术观察了吡咯并[1，2-a]吡嗪-1，4-二酮，六氢-3对MCF-7细胞的形态学损伤和增殖的影响。最后，使用Hoechst 33，342染色，通过荧光显微镜技术清楚地鉴定了吡咯并[1，2-a]吡嗪-1，4-二酮，六氢-3处理的MCF-7细胞的核浓缩和增加的坏死细胞。因此，我们的研究结果证明了吡咯并[1，2-a]皮拉嗪-1，4-二酮，六氢-3富集链霉菌秋吉GRG 6（KY 457710）作为一种潜在的抗MCF-7乳腺癌细胞的抗癌药物。(c)2020年，任作家。由Elsevier B. V.代表沙特国王大学出版。这是一个在CC BY许可证下的开放获取文章（http：//creativecommons.org/licenses/by/4.0/）。
The current study was concentrated to detection of anti-cancer compound from marine Streptomyces akiyoshiensis GRG 6 (KY457710) against MCF-7 breast cancer cells. The partial purification of TLC was moved three different active spots. Among the spots, the third spot of the extract was shown excellent anti-cancer properties against MCF-7 human breast cancer cells. The available anti-cancer compounds present in the partially purified extract was confirmed by GC-MS data interpretation. Three fractions of the compounds were purified by preparative HPLC and their respective spectrum was confirmed by analytical HPLC. Active anti-cancer fraction was separately purified by preparative HPLC and the cytotox-icity result was shown with 100 mg/mL concentration. The anti-cancer compound of pyrrolo [1,2-a] pyrazine-1,4-dione, hexahydro-3 was detected by LC-MS analysis after interpretation with GC-MS data. Further, the morphological damages and increased proliferation with more death cells of pyrrolo[1,2-a] pyrazine-1,4-dione, hexahydro-3 treated MCF-7 were observed by phase contrast and fluorescence microscope techniques. Finally, the nuclear condensation and increased necrotic cells of the pyrrolo[1, 2-a]pyrazine-1,4-dione, hexahydro-3 treated MCF-7 cells was clearly identified by fluorescence microscope techniques using Hoechst 33,342 stain. Therefore, our result was prove, the pyrrolo[1,2-a]pyra zine-1,4-dione, hexahydro-3 rich Streptomyces akiyoshiensis GRG 6 (KY457710) as a potential anticancer agent against MCF-7 breast cancer cells. (c) 2020 The Authors. Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).