The Rice Receptor-Like Kinases DWARF AND RUNTISH SPIKELET1 and 2 Repress Cell Death and Affect Sugar Utilization during Reproductive Development
The Rice Receptor-Like Kinases DWARF AND RUNTISH SPIKELET1 and 2 Repress Cell Death and Affect Sugar Utilization during Reproductive Development
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水稻受体样激酶 DWARF 和 RUNTISH SPIKELET1 和 2 抑制细胞死亡并影响生殖发育过程中的糖利用
DOI：
10.1105/tpc.16.00218
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发表时间：
2017-01-01
期刊：
影响因子：
11.6
通讯作者：
Sun, Ying
中科院分区：
文献类型：
--
作者：
Pu, Cui-Xia;Han, Yong-Feng;Sun, Ying
细胞与细胞之间的通讯精确地控制着生殖生长过程中新器官的产生。然而，在单子叶植物中介导发育信号的传感器分子知之甚少。在这里，我们报告的DWARF和RUNTISH小穗1（DRUS 1）和DRUS 2，两个密切相关的受体样激酶（RLKs），冗余控制生殖生长和发育的水稻（水稻）。一个drus 1 -1 drus 2双敲除突变体，但不是任何一个单一的突变体，表现出极端的矮化和不育的花序，窝藏不育小穗。该突变体的赤霉素途径没有受到损害。表达不同量的DRUS 1和2的突变体的表型比较显示，生殖生长需要一个阈值水平的DRUS 1/2蛋白。DRUS 1和2通过抑制蛋白酶介导的细胞降解并可能通过影响糖的利用或转化来维持细胞活力。在花药发育的后期，药室内壁的存活需要DRUS 1/2，这可能刺激UDP-葡萄糖焦磷酸化酶基因UGP 2的表达和花粉中淀粉的生物合成。与拟南芥的直系同源基因FERONIA不同，DRUS 1和2介导了一个基本的信号传导过程，这是细胞生存所必需的，并代表了CrRLK 1 L RLK亚家族的一种新的生物学功能。
Cell-to-cell communication precisely controls the creation of new organs during reproductive growth. However, the sensor molecules that mediate developmental signals in monocot plants are poorly understood. Here, we report that DWARF AND RUNTISH SPIKELET1 (DRUS1) and DRUS2, two closely related receptor-like kinases (RLKs), redundantly control reproductive growth and development in rice (Oryza sativa). A drus1-1 drus2 double knockout mutant, but not either single mutant, showed extreme dwarfism and barren inflorescences that harbored sterile spikelets. The gibberellin pathway was not impaired in this mutant. A phenotypic comparison of mutants expressing different amounts of DRUS1 and 2 revealed that reproductive growth requires a threshold level of DRUS1/2 proteins. DRUS1 and 2 maintain cell viability by repressing protease-mediated cell degradation and likely by affecting sugar utilization or conversion. In the later stages of anther development, survival of the endothecium requires DRUS1/2, which may stimulate expression of the UDP-glucose pyrophosphorylase gene UGP2 and starch biosynthesis in pollen. Unlike their Arabidopsis thaliana ortholog FERONIA, DRUS1 and 2 mediate a fundamental signaling process that is essential for cell survival and represents a novel biological function for the CrRLK1L RLK subfamily.