SEGMENTS OF GENOME OF VIRUSES CONTAINING DOUBLE-STRANDED RIBONUCLEIC ACID
SEGMENTS OF GENOME OF VIRUSES CONTAINING DOUBLE-STRANDED RIBONUCLEIC ACID
复制标题
DOI：
10.1016/0022-2836(70)90140-3
复制
发表时间：
1970-01-01
影响因子：
5.6
通讯作者：
FUKE, M
中科院分区：
文献类型：
--
作者：
FUJIIKAWATA, I;MIURA, KI;FUKE, M
Double-stranded RNA preparations which were obtained from cytoplasmic polyhedrosis virus of silkworm, rice dwarf virus and reovirus have been analyzed by polyacrylamide gel electrophoresis and ultraviolet scanning. Ten segments were recognized consistently for polyhedrosis virus and reovirus, and 12 for rice dwarf virus. The presence of such genome segments seems to be a common characteristic of double-stranded viral RNA. The size distribution of the genome segments is specific to each virus. The molecular weight of a segment ranged from 0.35 to 2.6 × 106for polyhedrosis virus, from 0.44 to 2.8 × 106for rice dwarf virus, and from 0.63 to 2.6 × 106for reovirus. The sum of the molecular weights of a set of these fragments (1.46 × 107for polyhedrosis RNA, 1.53 × 107for rice dwarf RNA and 1.49 × 107for reovirus RNA) corresponds to the calculated maximum molecular weight of RNA in a virion. Electron microscopic observation of the size distribution of these RNA preparations confirms the results of gel electrophoresis.
