A Synthetic Biology Approach to Engineering Living Photovoltaics.
A Synthetic Biology Approach to Engineering Living Photovoltaics.
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DOI：
10.1039/c7ee00282c
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发表时间：
2017-05-01
影响因子：
32.5
通讯作者：
Boghossian AA
中科院分区：
文献类型：
--
作者：
Schuergers N;Werlang C;Ajo-Franklin CM;Boghossian AA
活细胞与电子接受支架的电子接口能力对于下一代生物光伏技术的发展至关重要。虽然最近的研究集中在工程合成接口，可以最大限度地提高细胞和支架之间的电子通信，这种设备的效率是有限的细胞膜的低电导率。这篇评论提供了一个材料科学的角度应用互补的，合成生物学方法工程膜电极接口。它侧重于在细菌宿主细胞中引入外源细胞外电子转移途径背后的技术挑战，以及过去和未来为生物光伏应用设计具有人工电子输出能力的光合生物的努力。本文重点介绍了在模式宿主生物E。大肠杆菌，然后审查国家的最先进的生物光伏技术，使用未经修改的和生物工程的光合细菌，提高电子传输能力。一个热力学分析是用来提出一个积极可行的途径胞外电子传递工程蓝藻和确定代谢瓶颈服从蛋白质工程技术。基于这种分析，表达外源的基于蛋白质的电子导管的工程化光合生物产生6-10%的最大理论太阳能转换效率，而不考虑用于光捕获的额外生物工程优化。蓝藻细胞外电子传递途径的生物光伏应用
The ability to electronically interface living cells with electron accepting scaffolds is crucial for the development of next-generation biophotovoltaic technologies. Although recent studies have focused on engineering synthetic interfaces that can maximize electronic communication between the cell and scaffold, the efficiency of such devices is limited by the low conductivity of the cell membrane. This review provides a materials science perspective on applying a complementary, synthetic biology approach to engineering membrane-electrode interfaces. It focuses on the technical challenges behind the introduction of foreign extracellular electron transfer pathways in bacterial host cells and the past and future efforts to engineer photosynthetic organisms with artificial electron-export capabilities for biophotovoltaic applications. The article highlights advances in engineering protein-based, electron-exporting conduits in a model host organism, E. coli, before reviewing state-of-the-art biophotovoltaic technologies that use both unmodified and bioengineered photosynthetic bacteria with improved electron transport capabilities. A thermodynamic analysis is used to propose an energetically feasible pathway for extracellular electron transport in engineered cyanobacteria and identify metabolic bottlenecks amenable to protein engineering techniques. Based on this analysis, an engineered photosynthetic organism expressing a foreign, protein-based electron conduit yields a maximum theoretical solar conversion efficiency of 6–10% without accounting for additional bioengineering optimizations for light-harvesting. Engineering extracellular electron transfer pathways in cyanobacteria for biophotovoltaic applications