Dual multimodular class A penicillin-binding proteins in Mycobacterium leprae
Dual multimodular class A penicillin-binding proteins in Mycobacterium leprae
复制标题
麻风分枝杆菌中的双重多模块 A 类青霉素结合蛋白
DOI：
10.1128/jb.179.14.4627-4630.1997
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发表时间：
1997
影响因子：
3.2
通讯作者：
J. Ghuysen
中科院分区：
文献类型：
--
作者：
S. Lepage;P. Dubois;T. K. Ghosh;B. Joris;S. Mahapatra;M. Kundu;J. Basu;P. Chakrabarti;S. Cole;M. Nguyen;J. Ghuysen
The ponA gene of cosmid L222 of the Mycobacterium leprae genome library encodes a multimodular class A penicillin-binding protein (PBP), PBP1. The PBP, labelled with a polyhistidine sequence, has been produced in Escherichia coli, extracted from the membranes with 3-[(3-cholamidopropyl)-dimethylammonio]-1-propane-sulfonate (CHAPS) and purified by Ni2(+)-nitrilotriacetic acid-agarose chromatography. In contrast to the pon1-encoded class A PBP1, PBP1 undergoes denaturation at temperatures higher than 25 degrees C, it catalyzes acyl transfer reactions on properly structured thiolesters, and it binds penicillin with high affinity.
