Synthetic microbiota reveal priority effects and keystone strains in the Arabidopsis phyllosphere
Synthetic microbiota reveal priority effects and keystone strains in the Arabidopsis phyllosphere
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DOI：
10.1038/s41559-019-0994-z
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发表时间：
2019-10-01
影响因子：
16.8
通讯作者：
Vorholt, Julia A.
中科院分区：
文献类型：
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作者：
Carlstroem, Charlotte, I;Field, Christopher M.;Vorholt, Julia A.
包括植物在内的多细胞生物被微生物定植，其中一些微生物对生长和健康有益。建立植物微生物群的组装规则尚不清楚，它们的成员相互作用的程度也不清楚。本研究采用62种原生菌株合成群落接种拟南芥，进行了退群和后期引种实验，考察了到达顺序对群落结构的影响。作为一个读数，我们跟踪了单个植物层层中所有菌株的相对丰度。我们的研究结果表明，社区聚集在历史上是偶然的，并受到优先效应的影响。缺失的菌株可能在不同程度上侵入已经建立的微生物群，这些微生物群本身具有耐药性，并且基本上不受后来者的影响。此外，我们的研究结果表明，变形菌门（鞘单胞菌、根瘤菌）和放线菌门（微细菌、红球菌）作为关键物种，对群落结构的影响潜力最大。
Multicellular organisms, including plants, are colonized by microorganisms, some of which are beneficial to growth and health. The assembly rules for establishing plant microbiota are not well understood, and neither is the extent to which their members interact. We conducted drop-out and late introduction experiments by inoculating Arabidopsis thaliana with synthetic communities from a resource of 62 native bacterial strains to test how arrival order shapes community structure. As a read-out we tracked the relative abundance of all strains in the phyllosphere of individual plants. Our results showed that community assembly is historically contingent and subject to priority effects. Missing strains could, to various degrees, invade an already established microbiota, which was itself resistant and remained largely unaffected by latecomers. Additionally, our results indicate that individual strains of Proteobacteria (Sphingomonas, Rhizobium) and Actinobacteria (Microbacterium, Rhodococcus) have the greatest potential to affect community structure as keystone species.