Guanine nucleotides inhibit agonist-stimulated arachidonic acid release in both intact and saponin-permeabilized human platelets.
Guanine nucleotides inhibit agonist-stimulated arachidonic acid release in both intact and saponin-permeabilized human platelets.
复制标题
鸟嘌呤核苷酸抑制完整和皂苷渗透的人血小板中激动剂刺激的花生四烯酸释放。
DOI：
10.1016/0014-5793(88)80107-8
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发表时间：
1988
期刊：
影响因子：
3.5
通讯作者：
Halenda,SP
中科院分区：
文献类型：
--
作者：
Rehm,AG;Wu,H;Halenda,SP
在完整和皂苷透化的人血小板中研究了鸟嘌呤核苷酸对花生四烯酸（AA）释放的影响。虽然GTP[S]本身引起透化细胞中AA释放的刺激，但GTP[S]、GDP[S]、GTP、ATP和其他核苷酸抑制完整血小板以及透化血小板中响应于凝血酶和其他激动剂的AA释放。核苷酸对激动剂刺激的AA释放的抑制作用通过加入ADP而部分减弱，并且通过预先刺激血小板以排出含ADP的致密颗粒而消除。这些结果表明：（i）释放的ADP在激动剂刺激的血小板AA释放中起着重要的促进作用，（ii）鸟嘌呤核苷酸可以通过细胞外作用调节血小板活化，这与它们对G蛋白的作用不同。
The effects of guanine nucleotides on arachidonic acid (AA) release were studied in intact and saponin‐permeabilized human platelets. While GTP[S] itself caused a stimulation of AA release in permeabilized cells, GTP[S], GDP[S], GTP, ATP and other nucleotides inhibited AA release in response to thrombin and other agonists in intact, as well as permeabilized platelets. Inhibition of agonist‐stimulated AA release by nucleotides was partially attenuated by addition of ADP, and was abolished by prior stimulation of platelets to discharge the ADP‐containing dense granules. These results suggest: (i) that released ADP plays an important contributory role in agonist‐stimulated platelet AA release, and (ii) that guanine nucleotides can modulate platelet activation through an extracellular action which is distinct from their effects on G‐proteins.