Diabetes-Invoked High-Density Lipoprotein and Its Association With Coronary Artery Disease in Patients With Type 2 Diabetes Mellitus
Diabetes-Invoked High-Density Lipoprotein and Its Association With Coronary Artery Disease in Patients With Type 2 Diabetes Mellitus
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糖尿病引起的高密度脂蛋白及其与 2 型糖尿病患者冠状动脉疾病的关系
DOI：
10.1016/j.amjcard.2016.08.044
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发表时间：
2016-12-01
影响因子：
2.8
通讯作者：
Zhang, Rui Yan
中科院分区：
文献类型：
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作者：
Sun, Jia Teng;Liu, Yan;Zhang, Rui Yan
虽然高密度脂蛋白（HDL）可以表现出抗炎特性，但这些有效的活性可能会变得不足，甚至在各种病理生理状态下转化为促炎作用。我们研究了2型糖尿病（DM）患者高密度脂蛋白（HDL）对单核细胞炎症反应的影响及其与冠心病（CAD）的关系。HDL分离自患有（n = 61）或不患有（n = 31）CAD（直径狭窄50%）的DM患者和健康对照（n = 40）。将人外周血单个核细胞与HDL孵育，并通过外周血单个核细胞中肿瘤坏死因子-α（TNF-α）的分泌来确定HDL的促炎能力。与对照HDL相比，人单核细胞对糖尿病HDL的应答中TNF-α的分泌显著增加。值得注意的是，来自患有CAD的DM患者的HDL刺激单核细胞中TNF-α的释放的程度大于来自没有CAD的那些患者的HDL。多元线性回归分析显示，HDL的促炎作用与糖尿病病程、血红蛋白A1 c、血清超敏C反应蛋白（hs-CRP）水平和肾小球滤过率（GFR）降低独立相关。此外，HDL的促炎能力是DM患者存在CAD的重要预测因子。(C)2016 Elsevier Inc. All rights reserved.
Although high-density lipoprotein (HDL) can exhibit anti-inflammatory properties, these potent activities can become deficient and even transform into proinflammatory effects under various pathophysiological states. We investigated the effect of diabetic HDL on the inflammatory response in human monocytes and its relation to the existence of coronary artery disease (CAD) in patients with type 2 diabetes mellitus (DM). HDL was isolated from DM patients with (n = 61) or without (n = 31) CAD (diameter stenosis 50%) and healthy controls (n = 40). Human peripheral blood mononuclear cells were incubated with HDL and the proinflammatory ability of HDL was determined by tumor necrosis factor-alpha (TNF-alpha) secretion in peripheral blood mononuclear cells. Secretion of TNF-alpha in human monocytes in response to diabetic HDL was significantly increased compared with that of the control HDL. Of note, HDL from DM patients with CAD stimulated the release of TNF-alpha in monocytes to a greater extent than that of HDL from those without CAD. Multiple linear regression analysis showed that the proinflammatory ability of HDL was independently associated with diabetes duration, hemoglobin A1c, serum levels of high-sensitivity C-reactive protein (hs-CRP) and reduced glomerular filtration rate (GFR). Furthermore, the proinflammatory ability of HDL was a significant predictor for the presence of CAD in patients with DM. (C) 2016 Elsevier Inc. All rights reserved.