Role of lumican in the corneal epithelium during wound healing
Role of lumican in the corneal epithelium during wound healing
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DOI：
10.1074/jbc.275.4.2607
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发表时间：
2000-01-28
影响因子：
4.8
通讯作者：
Kao, WWY
中科院分区：
文献类型：
--
作者：
Saika, S;Shiraishi, A;Kao, WWY
Lumican作为角膜中的硫酸角质素蛋白聚糖调节胶原基质组装，并且还作为糖蛋白存在于其他器官的结缔组织和胚胎角膜基质中。在正常的未受伤的角膜中，基质角膜细胞表达Lumican。我们的数据表明，受伤的小鼠角膜上皮异位和瞬时表达lumican在伤口愈合的早期阶段，这表明一个潜在的lumican功能无关的胶原纤维生成的调节，如调节上皮细胞粘附或迁移。在培养的小鼠眼睛中发现抗Lumican抗体延迟角膜上皮伤口愈合。与Lum（+/-）小鼠相比，Lum（-/-）小鼠中角膜上皮损伤的愈合显著延迟。这些观察结果表明，在受损上皮中表达的Lumican可以调节细胞行为，例如粘附或迁移，从而有助于角膜上皮伤口愈合。
Lumican regulates collagenous matrix assembly as a keratan sulfate proteoglycan in the cornea and is also present in the connective tissues of other organs and embryonic corneal stroma as a glycoprotein. in normal unwounded cornea, lumican is expressed by stromal keratocytes. Our data show that injured mouse corneal epithelium ectopically and transiently expresses lumican during the early phase of wound healing, suggesting a potential lumican functionality unrelated to regulation of collagen fibrillogenesis, e.g. modulation of epithelial cell adhesion or migration. An anti-lumican antibody was found to retard corneal epithelial wound healing in cultured mouse eyes. Healing of a corneal epithelial injury in Lum(-/-) mice was significantly delayed compared with Lum(+/-) mice. These observations indicate that lumican expressed in injured epithelium may modulate cell behavior such as adhesion or migration, thus contributing to corneal epithelial wound healing.