Coexistence of gastric‐ and intestinal‐type endocrine cells in gastric and intestinal mixed intestinal metaplasia of the human stomach
Coexistence of gastric‐ and intestinal‐type endocrine cells in gastric and intestinal mixed intestinal metaplasia of the human stomach
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DOI：
10.1111/j.1440-1827.2005.01809.x
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发表时间：
2005-04
影响因子：
2.2
通讯作者：
T. Otsuka;T. Tsukamoto;T. Mizoshita;K. Inada;Y. Takenaka;S. Kato;Y. Yamamura;K. Miki;M. Tatematsu
中科院分区：
文献类型：
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作者：
T. Otsuka;T. Tsukamoto;T. Mizoshita;K. Inada;Y. Takenaka;S. Kato;Y. Yamamura;K. Miki;M. Tatematsu
人胃中的肠化生（IM）以前被分为胃和肠混合型（GI-IM）和纯肠表型（I-IM）。对 3034 个与慢性胃炎相关的腺管中的粘液和内分泌细胞的表型进行了评估。在幽门区，胃泌素、生长抑素等胃内分泌细胞标志物的相对表达量从仅有胃粘膜细胞表型（G型）的腺管到GI-IM再向I-IM逐渐下降，而肠内分泌细胞标志物高血糖素、抑胃多肽（GIP）和胰高血糖素样肽-1（GLP-1）的相对表达量呈负相关。在胃底区域，假幽门腺和 GI-IM 腺中出现胃泌素阳性细胞，而 I-IM 腺不具有任何胃泌素阳性细胞，表明存在独特的肠化途径。双重染色显示胃泌素和 GLP-1 阳性细胞在同一腺体中共存，有时在 GI-IM 腺体中的同一细胞中共存。这些结果表明，内分泌细胞的表型与粘液细胞的表型一致，并支持正常腺体和肌内腺体中所有不同类型的粘液细胞和内分泌细胞可能源自每个腺体中的单个祖细胞的概念。
Intestinal metaplasia (IM) in the human stomach has previously been classified into a gastric and intestinal mixed (GI‐IM) and a solely intestinal phenotype (I‐IM). The phenotypes of mucous and endocrine cells were evaluated in 3034 glandular ducts associated with chronic gastritis. In the pyloric region, the relative expression of gastric endocrine cell markers, such as gastrin and somatostatin, decreased gradually from glandular ducts with only gastric mucous cell phenotype (G type) to GI‐IM toward I‐IM, while that of the intestinal endocrine cell markers, glicentin, gastric inhibitory polypeptide (GIP), and glucagon‐like peptide‐1 (GLP‐1) was inversely correlated. In the fundic region, gastrin‐positive cells emerged in the pseudo‐pyloric and GI‐IM glands, whereas I‐IM glands did not possess any gastrin‐positive cells, suggesting the presence of a distinct pathway of intestinalization. Double staining revealed coexistence of gastrin‐ and GLP‐1‐positive cells in the same gland and occasionally in the same cell in GI‐IM glands. These results suggest that the phenotypes of endocrine cells are in line with those for mucous counterparts and support the concept that all of the different types of mucous and endocrine cells in normal and IM glands might be derived from a single progenitor cell in each gland.