Adsorption Behavior of Cytochrome c, Myoglobin and Hemoglobin in a Quartz Surface Probed Using Slab Optical Waveguide (SOWG) Spectroscopy
Adsorption Behavior of Cytochrome c, Myoglobin and Hemoglobin in a Quartz Surface Probed Using Slab Optical Waveguide (SOWG) Spectroscopy
复制标题
使用平板光波导 (SOWG) 光谱法探测石英表面中细胞色素 c、肌红蛋白和血红蛋白的吸附行为
作者：
J. Santos;N. Matsuda;Z. Qi;Takamitsu Yoshida;A. Takatsu;Kenji Kato
利用平板光波导（SOWG）技术研究了细胞色素c、肌红蛋白和血红蛋白在石英表面的吸附行为。使用棱镜耦合多色可见光传播到石英波导内的全反射，倏逝波吸收的实时监测揭示了强烈的蛋白质浓度，溶液pH值和离子强度的蛋白质表面相互作用的依赖性。对于研究的三种蛋白质，吸光度-体积浓度比在低体积浓度下较高，在较高浓度下降低。对于细胞色素c和肌红蛋白，吸光度接近极限值，但缓冲血红蛋白令人惊讶地没有显示形成信号平台的任何指示。此外，缓慢引入蛋白质到溶液中减少了每单位面积的总吸附量。这些观察结果表明，在石英表面吸附后的蛋白质分子的可能的构象转变。对于体积较大的蛋白质，血红蛋白，吸附到石英表面上的磷酸盐缓冲液的存在下，增强，而相反的效果，观察到较小的细胞色素c和肌红蛋白分子。pH研究的结果与蛋白质和石英表面的等电数据预测的静电相互作用一致。
Slab optical waveguide (SOWG) spectroscopy was used to observe the adsorption behavior of three important heme proteins, namely cytochrome c , myoglobin and hemoglobin, in a quartz surface. Using prism-coupled polychromatic visible light propagated into a quartz waveguide by internal total reflection, the real-time monitoring of evanescent wave absorption revealed a strong dependence of the protein-surface interaction on the protein concentration, the solution pH and the ionic strength. For the three proteins studied, the absorbance-bulk concentration ratio was higher at low bulk concentrations, and decreased at higher concentrations. For cytochrome c and myoglobin, the absorbance approached a limiting value, but buffered hemoglobin surprisingly did not show any indication of forming a signal plateau. Moreover, the slow introduction of protein into the solution lessened the total adsorbed amount per unit area. These observations suggested a possible conformational transition of the protein molecules at the quartz surface after adsorption. For a bulkier protein, hemoglobin, adsorption onto the quartz surface was enhanced in the presence of a phosphate buffer, while the opposite effect was observed for the smaller cytochrome c and myoglobin molecules. The results of pH studies concurred with the electrostatic interactions predicted from the isoelectric data of proteins and the quartz surface.
影响因子：
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作者：
V. Hlady;Jos Buijs;Herbert P. Jennissen
通讯作者：
V. Hlady;Jos Buijs;Herbert P. Jennissen
影响因子：
3.4
作者：
Edmiston, PL;Saavedra, SS
通讯作者：
Saavedra, SS