Quantitative comparison of the HSV-1 and HSV-2 transcriptomes using DNA microarray analysis
Quantitative comparison of the HSV-1 and HSV-2 transcriptomes using DNA microarray analysis
复制标题
DOI：
10.1016/j.virol.2005.12.036
复制
发表时间：
2006-04-25
期刊：
影响因子：
3.7
通讯作者：
Wagner, EK
中科院分区：
文献类型：
--
作者：
Aguilar, JS;Devi-Rao, GV;Wagner, EK
人类疱疹病毒1型和2型基因组具有高度的序列同一性；然而，它们在自然人类宿主以及动物宿主和细胞培养中表现出重要的病理差异。在这里，我们报告了使用平行感染和使用与同源HSV-2转录物高效杂交的HSV-1探针进行微阵列分析的每种病毒类型（其转录组）转录的时间和相对丰度谱的比较分析。我们已经证实，同源转录本属于同一动力学类；然而，4种转录本(U(L)4、U(L)29、U（L)30和U(L)31）在两种病毒感染中的积累时间模式不同。有趣的是，这些转录物的蛋白产物都参与了核组织和病毒DNA定位。我们讨论了这些发现的相关性，以及它们是否可能在HSV-1和HSV-2的病理差异中发挥潜在作用。(c) 2006爱思唯尔公司版权所有。
The genomes of human herpes virus type-1 and type-2 share a high degree of sequence identity; yet, they exhibit important differences in pathology in their natural human host as well as in animal host and cell cultures. Here, we report the comparative analysis of the time and relative abundance profiles of the transcription of each virus type (their transcriptomes) using parallel infections and microarray analysis using HSV-1 probes which hybridize with high efficiency to orthologous HSV-2 transcripts. We have confirmed that orthologous transcripts belong to the same kinetic class; however, the temporal pattern of accumulation of 4 transcripts (U(L)4, U(L)29, U(L)30, and U(L)31) differs in infections between the two virus types. Interestingly, the protein products of these transcripts are all involved in nuclear organization and viral DNA localization. We discuss the relevance of these findings and whether they may have potential roles in the pathological differences of HSV-1 and HSV-2. (c) 2006 Elsevier Inc. All rights reserved.