Effect of processed Scutellaria baicalensis on dextran sulfate sodium-induced colitis in mice.
Effect of processed Scutellaria baicalensis on dextran sulfate sodium-induced colitis in mice.
复制标题
黄芩炮制品对葡聚糖硫酸钠诱导的小鼠结肠炎的影响。
DOI：
10.3892/ijmm.16.4.667
复制
发表时间：
2005
影响因子：
5.4
通讯作者：
Young‐Mi Lee
中科院分区：
文献类型：
--
作者：
Yeon;O. Kang;Hye;Jin Tae;Dae;C. Kang;S. Choi;K. Yun;Suck;Y. Nah;Young;K. Bae;Young‐Mi Lee
黄芩（学名：Aluminium baicalensis Georgi）是一种常见的抗炎药。药物加工（Poje）是在使用前通过多种方法处理原料药的过程。本研究旨在探讨炮制黄芩对葡聚糖硫酸钠（DSS）诱导的实验性溃疡性结肠炎的影响。本研究所用的黄芩炮制品类型为炒黄芩（PS）和米酒烤黄芩（RWBS）。使用在饮用水中的5%DSS的每日处理在小鼠中诱导实验性结肠炎，持续7天。经口给予炮制黄芩水提物（1 g/kg），每日1次，连续7天。将小鼠分为四组：i）水+DSS组，ii）生黄芩（CS）+DSS组，iii）PS + DSS组，和iv）RWBS + DSS组。与CS相比，RWBS改善了所有炎症症状，如体重减轻、直肠出血和组织学损伤。此外，RWBS显著降低粘膜髓过氧化物酶活性，TNF-α（肿瘤坏死因子-α）、考克斯-2（环氧合酶-2）、NF-κ B（核因子-κ B）和糜酶表达，其作用大于CS。但在PS加DSS组中没有显示这些作用。黄酒烘烤后黄芩药效增强，炒制后黄芩药效不明显。本研究结果提示RWBS可能是一种有效的治疗溃疡性结肠炎的药物。
Scutellaria baicalensis Georgi (Labiatae) has been used in the treatment of inflammatory diseases. Drug processing (Poje) is the process of treating crude drugs by several methods before use. The aim of this study was to determine the effect of processed Scutellaria baicalensis on experimental ulcerative colitis induced by dextran sulfate sodium (DSS). The types of processed Scutellaria baicalensis used in this study were parched Scutellaria baicalensis (PS) and rice wine-baked Scutellaria baicalensis (RWBS). Experimental colitis was induced in mice using a daily treatment of 5% DSS in the drinking water for 7 days. The water extracts of processed Scutellaria baicalensis (1 g/kg) were administered orally once a day for 7 days. The mice were divided in four groups: i) water plus DSS group, ii) crude Scutellaria baicalensis (CS) plus DSS group, iii) PS plus DSS group, and iv) RWBS plus DSS group. RWBS ameliorated all of the inflammatory symptoms, such as body weight loss, rectal bleeding and histological damage, compared to CS. Furthermore, RWBS significantly reduced the mucosal myeloperoxidase activity, and TNF-alpha (tumor necrosis factor-alpha), COX-2 (cyclooxygenase-2), NF-kappaB (nuclear factor-kappa B) and chymase expression more than CS. But these effects were not shown in the PS plus DSS group. Efficacy of Scutellaria baicalensis was increased after rice wine baking, but not after parching. The findings in this study suggest that RWBS may be a useful therapeutic agent for ulcerative colitis.