Characteristics of non-typhoidal southwestern Salmonella isolates from human and broiler-chickens in Seoul, Korea
Characteristics of non-typhoidal southwestern Salmonella isolates from human and broiler-chickens in Seoul, Korea
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DOI：
10.3346/jkms.2007.22.5.773
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发表时间：
2007-10-01
影响因子：
4.5
通讯作者：
Kim, Woo Joo
中科院分区：
文献类型：
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作者：
Cheong, Hee Jin;Lee, Yeon Joo;Kim, Woo Joo
非伤寒沙门氏菌是一种重要的共生微生物。本研究的目的是通过药物敏感性分析和分子分型，确定家畜NTS分离株与人NTS分离株之间的流行病学关系。我们对36株人临床分离株和家畜分离株进行了血清型测定，对8种抗生素进行了药敏试验，并对分离的新城疫杆菌进行了分子分型。脉冲场凝胶电泳(PFGE)。在人类分离株中，肠炎沙门氏菌是最常见的血清型(17株，占47.2%)，鼠伤寒沙门氏菌次之(8株，占22.2%)。在家畜分离株中，鼠伤寒沙门氏菌是最常见的血清型(15株，占23.44%)，其次是肠炎沙门氏菌(14株，占21.88%)。肉鸡-鸡NTS分离株对氨苄西林和四环素的耐药率各为50%(32株/)。无人畜NTS分离株对环丙沙星、TMP-SMX或头孢曲松耐药。然而，人和家畜NTS分离株中19.4%(7/36)和46.8%(30/)对奈立地酸(MIC>；=16 mg/mL)耐药。来自人和肉鸡NTS分离株的三种相同的PFGE分子类型的存在表明了从牲畜向人传播的可能性。
Non-typhoidal Salmonella (NTS) is an important commensal microorganism. The purpose of this study was to determine the epidemiological relation between NTS isolates from livestock and NTS isolates from human by analyzing antimicrobial susceptibilities and performing molecular typing. We determined the serotypes of 36 human clinical isolates and 64 livestock isolates, performed antimicrobial susceptibility testing against 8 antibiotics, and determined the molecular types of isolated NTS spp. by pulsed field gel electrophoresis (PFGE). In human isolates, S. enteritidis was the most common serotype (17 isolates; 47.2%) and S. typhimurium the second most (8 isolates; 22.2%). In livestock isolates, S. typhimurium was the most common serotype (15 isolates; 23.44%), and S. enteritidis was the second most (14 isolates; 21.88%). Ampicillin and tetracycline resistance were 50% (32/64 isolates) each among broiler-chicken NTS isolates. No human or livestock NTS isolates showed resistance to ciprofloxacin, TMP-SMX, or ceftriaxone. However, 19.4% (7/36) and 46.8% (30/64) of the human and livestock NTS isolates were resistant to nalidixic acid (MIC >= 16 mg/mL), respectively. The presence of the three identical PFGE molecular types from human and broiler-chicken NTS isolates suggests the possibility of transmission from livestock to humans.