The Putative Cellodextrin Transporter-like Protein CLP1 Is Involved in Cellulase Induction in Neurospora crassa
The Putative Cellodextrin Transporter-like Protein CLP1 Is Involved in Cellulase Induction in Neurospora crassa
复制标题
假定的纤维糊精转运蛋白样蛋白 CLP1 参与粗糙脉孢菌中的纤维素酶诱导
DOI：
10.1074/jbc.m114.609875
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发表时间：
2015-01-09
影响因子：
4.8
通讯作者：
Ma, Yanhe
中科院分区：
文献类型：
--
作者：
Cai, Pengli;Wang, Bang;Ma, Yanhe
粗糙脉孢菌（Neurosporacrassa）是近年来研究纤维素酶诱导的一个新体系。在这里，我们发现了一种新的膜蛋白，纤维糊精转运蛋白样蛋白1（CLP 1; NCU 05853），一种假定的纤维糊精转运蛋白样蛋白，是N.粗鲁。虽然CLP 1蛋白不能运输纤维糊精，但在纤维二糖和Avicel上都发现了该蛋白对纤维素酶诱导的抑制作用。菌株Delta 3 β G中的纤维糊精转运蛋白cdt 2和clp 1的共破坏形成菌株CPL 7。在纤维二糖的诱导下，与Delta 3 β G相比，CPL 7中的纤维素酶产量提高了6.9倍。我们还表明，CLP 1抑制纤维素酶的表达发生通过抑制纤维糊精转运蛋白的表达，特别是cdt 1的表达。产超纤维素酶菌株CPL 7的转录组分析表明，纤维素酶表达机制显着刺激，纤维素酶基因，包括诱导转运蛋白和主要的转录调节因子。
Neurospora crassa recently has become a novel system to investigate cellulase induction. Here, we discovered a novel membrane protein, cellodextrin transporter-like protein 1 (CLP1; NCU05853), a putative cellodextrin transporter-like protein that is a critical component of the cellulase induction pathway in N. crassa. Although CLP1 protein cannot transport cellodextrin, the suppression of cellulase induction by this protein was discovered on both cellobiose and Avicel. The co-disruption of the cellodextrin transporters cdt2 and clp1 in strain Delta 3 beta G formed strain CPL7. With induction by cellobiose, cellulase production was enhanced 6.9-fold in CPL7 compared with Delta 3 beta G. We also showed that the suppression of cellulase expression by CLP1 occurred by repressing the expression of cellodextrin transporters, particularly cdt1 expression. Transcriptome analysis of the hypercellulase-producing strain CPL7 showed that the cellulase expression machinery was dramatically stimulated, as were the cellulase enzyme genes including the inducer transporters and the major transcriptional regulators.