Methods for isolating circulating epithelial cells and criteria for their classification as carcinoma cells
Methods for isolating circulating epithelial cells and criteria for their classification as carcinoma cells
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DOI：
10.1080/14653240510027082
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发表时间：
2005-05-01
期刊：
影响因子：
4.5
通讯作者：
Gebauer, G
中科院分区：
文献类型：
--
作者：
Fehm, T;Solomayer, EF;Gebauer, G
开发用于上皮来源的循环肿瘤细胞（CTC）的分离和表征的新型测定方法提供了用于非侵入性收集与癌症的诊断、演变和治疗相关的临床信息的标记物的潜力。在目前用于分析CTC的众多技术中，基于载玻片的测定可能是最常见的。虽然传统的结合免疫细胞化学/明场显微镜系统仍然是最常用的，荧光为基础的分析越来越重要。这部分是因为荧光显微镜分析载玻片CTC可以同时提供细胞遗传学以及形态学和多种表型信息。特别是，载玻片CTC的荧光显微镜分析可以准确地确定复发性疾病患者染色体水平的遗传变化。更重要的是，通过确定CTC中的遗传畸变，可以选择最有可能从给定治疗中获益的患者。该技术的潜力已经通过采用荧光原位杂交（FISH）方法来测量乳腺癌患者组织中HER 2/neu基因的表达，以确定最有可能对曲妥珠单抗靶向治疗有反应的患者的特定目的而得到证实。在这里，我们审查的主要方法用于制备和分析的载玻片为基础的测定。
Novel assay methods developed for the isolation and characterization of circulating tumor cells (CTC) of epithelial origin offer the potential of markers for the non-invasive gathering of clinical information relevant to the diagnosis, evolution and treatment of carcinoma. Of the numerous techniques currently used to analyze CTC, slide-based assays are perhaps the most common. While traditional combined immunocytochemical/brightfield microscopy systems continue to be the most frequently employed, fluorescence-based analysis is gaining in importance. This is partly because fluorescence microscopy analysis of slide-mounted CTC can provide simultaneously cytogenetic as well as morphologic and multiple phenotypic information. In particular, fluorescence microscopy analysis of slide-mounted CTC can accurately determine genetic changes at the chromosomal level in patients with recurrent disease. More importantly, by identifying genetic aberrations in CTC, it becomes possible to choose those patients most likely to benefit from a given treatment. The potential of this technique has already been demonstrated by employing fluorescence in situ hybridization (FISH) methods to measure expression of the HER2/neu gene in tissue from patients with breast carcinoma for the specific purpose of identifying those patients most likely to respond to Trastuzumab targeted therapy. Here, we review the major methodologies used in the preparation and analysis of the slide-based assays.