Langmuir Trough Study of the Interactions of Tear Mimetic Eyedrop Formulation with Human Meibum Films
Langmuir Trough Study of the Interactions of Tear Mimetic Eyedrop Formulation with Human Meibum Films
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DOI：
10.3390/app122312095
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发表时间：
2022-12-01
影响因子：
2.7
通讯作者：
Georgiev, Georgi As.
中科院分区：
文献类型：
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作者：
Eftimov, Petar;Yokoi, Norihiko;Georgiev, Georgi As.
睑板腺疾病与导致泪膜不稳定的睑脂（MGS）的定量或定性缺陷相关。因此，对可以补充MGS并恢复其在空气/泪液表面的性能的眼用纳米乳液存在巨大需求。Rohto Dry Aid（RDA）利用TEARSHIELD TECHNOLOGY（TM）实现非极性和极性脂质分子的复杂油相。因此，RDA与MGS表面膜的相互作用值得进一步研究，因为它们可以提供有价值的见解（i）纳米乳液治疗作用背后的机制和（ii）设计新型眼用制剂。在朗缪尔槽的空气-水表面形成了伪二元meibum/RDA膜。表面压力-面积等值线和应力松弛分别用于探测层（i）循环后的重组和（ii）分解弹性。通过布鲁斯特角显微镜观察膜形态，并探讨添加RDA的睑脂的铺展特性。纳米乳液油相的不同成分补充了睑板层的非极性和极性脂质成分，这导致MGS双相膜结构的连续性增强，并促进了MGS铺展和粘弹性。纳米乳剂作为MGS导向的干眼治疗的有效工具值得进一步研究。
Meibomian gland disease is associated with quantitative or qualitative deficiencies of meibum (MGS) that result in tear film instability. Thus, there is great demand for ophthalmic nanoemulsions that can replenish MGS and recover its performance at the air/tear surface. Rohto Dry Aid (RDA) utilizes TEARSHIELD TECHNOLOGY (TM) implementing a complex oil phase of non-polar and polar lipid-like molecules. Therefore, the interactions of RDA with MGS surface films deserve further study as they may provide valuable insights (i) into the mechanisms behind the nanoemulsion therapeutic action and (ii) for the design of novel ophthalmic formulations. Pseudobinary meibum/RDA films were formed at the air-water surface of the Langmuir trough. Surface pressure-area isocycles and stress relaxations were employed to probe the layer (i) reorganization upon cycling and (ii) dilatational elasticity, respectively. Film morphology was accessed by Brewster angle microscopy and the spreading properties of RDA-supplemented meibum were also probed. The diverse ingredients of the nanoemulsion oil phase complemented the non-polar and polar lipid constituents of the meibomian layers, which resulted in enhanced continuity of the MGS duplex film structure and facilitated the MGS spread and viscoelasticity. Nanoemulsions deserve further study as a potent tool for MGS-oriented therapy for dry eyes.