[Effect of electroacupuncture with different frequencies on hindlimb locomotor function and expression of LC3, Beclin 1 and cleaved Caspase-3 proteins in spinal cord injury rats].
[Effect of electroacupuncture with different frequencies on hindlimb locomotor function and expression of LC3, Beclin 1 and cleaved Caspase-3 proteins in spinal cord injury rats].
复制标题
DOI：
10.13702/j.1000-0607.190068
复制
发表时间：
2019-09-25
期刊：
影响因子：
--
通讯作者：
Xu, Bo
中科院分区：
文献类型：
--
作者：
Luo, Hui-Ying;Zeng, Zhi-Wei;Xu, Bo
目的：探讨不同频率电针（EA）对脊髓损伤（SCI）大鼠运动功能及自噬相关蛋白微管相关蛋白轻链3（LC3）等表达的影响，揭示其潜在机制，为临床应用提供参考。 方法：将100只雄性SD大鼠随机分为假手术组、模型组、2 Hz-EA、100 Hz-EA和2组。 Hz/100 Hz-EA组（每组n=20）。将EA（2 Hz、100 Hz或2 Hz/100 Hz，0.4-0.5-0.6 mA每10分钟增加0.1 mA）应用于“华佗佳吉”（EX-HN1、T9和T11）30分钟，每天一次，连续7天。分别采用Basso、Beattie和Bresnahan(BBB)运动评定量表、斜面试验和足底印迹试验评价大鼠后肢运动功能。分别采用荧光玉B（FJB）和尼氏染色观察脊髓组织的组织学变化和神经元凋亡。 Western blot检测脊髓中LC3、Beclin 1和Cleaved Caspase-3蛋白的表达。结果：造模后第1、3、7天BBB评分和斜面角度均显着降低（P
OBJECTIVE: To investigate the effect of different frequencies of electroacupuncture (EA) on motor function and expression of autophagy-related proteins microtubule-associated protein light chain 3 (LC3), etc. in spinal cord injury (SCI) rats, so as to reveal its underlying mechanisms and provide a reference for clinical application.METHODS: A total of 100 male SD rats were randomly divided into sham control, model, 2 Hz-EA, 100 Hz-EA and 2 Hz/100 Hz-EA groups(n=20 in each group). EA (2 Hz, 100 Hz or 2 Hz/100 Hz, 0.4-0.5-0.6 mA increased by 0.1 mA every10 min) was applied to "HuatuoJiaji" (EX-HN1, T9 and T11) for 30 min, once a day for 7 days. The rat's hindlimb motor function was assessed by using Basso, Beattie and Bresnahan(BBB) locomotor rating scale, inclined plane test and plantar imprinting test, separately. The histological changes and neuronal apoptosis of the spinal cord tissue were observed by Fluoro-Jade B (FJB) and Nissl staining, respectively. The expression of LC3, Beclin 1 and cleaved Caspase-3 proteins in the spinal cord was detected by Western blot.RESULTS: After modeling, the BBB scores and the angles of inclined plane on day 1, 3 and 7 were significantly decreased (P