Selective solid-phase extraction of a triterpene acid from a plant extract by molecularly imprinted polymer
Selective solid-phase extraction of a triterpene acid from a plant extract by molecularly imprinted polymer
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DOI：
10.1016/j.talanta.2007.11.037
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发表时间：
2008-04-15
期刊：
影响因子：
6.1
通讯作者：
Haupt, Karsten
中科院分区：
文献类型：
--
作者：
Claude, Berengere;Morin, Philippe;Haupt, Karsten
以甲基丙烯酸为功能单体，乙二醇二甲基丙烯酸酯为交联剂，氯仿为致孔溶剂，齐烷三萜化合物（18- -甘草次酸）为印迹分子（模板），采用热聚合法制备了分子印迹聚合物（MIP）。通过平衡配体结合实验来评价MIP相对于非印迹聚合物（NIP）的性能。优化固相萃取工艺（CHCl3为洗涤溶剂，MeOH为洗脱溶剂），通过对比NIP（5%）和MIP（97%）的回收率，证实印迹成功。测定MIP对18- β -甘草次酸的结合能力为0.94 mg g（-1）。选择4种结构相关的齐墩烷三萜（18- α -甘草次酸、齐墩果酸、棘囊酸、红二醇）进行MIP选择性评价。实验数据说明了官能团对三萜骨架的影响。采用高效液相色谱法对一种植物提取物进行固相萃取。然而，在洗涤步骤中，CHCl3被ACN取代，以抑制极性矩阵成分引起的非特异性相互作用。从甘草根水解液中选择性提取18- β -甘草次酸，提取率达到98%。(c) 2007 Elsevier B.V.版权所有
A molecularly imprinted polymer (MIP) has been prepared by a thermal polymerisation method using methacrylic acid as functional monomer, ethylene glycol dimethacrylate as cross-linking agent, chloroform as porogenic solvent and an oleanane triterpene compound (18-beta-glycyrrhetinic acid) as imprinted molecule (template).Equilibrium ligand binding experiments were done to assess the performance of the MIP relative to non-imprinted polymer (NIP).After optimisation of SPE protocol (CHCl3 as washing solvent and MeOH as elution solvent), successful imprinting was confirmed by comparison of the recoveries between NIP (5%) and MIP (97%) cartridges. The binding capacity of the MIP for 18-beta-glycyrrhetinic acid was determined to be 0.94 mg g(-1).Four structurally related oleanane triterpenes (18-alpha-glycyrrhetinic acid, oleanolic acid, echinocystic acid, erythrodiol) were selected to assess the MIP selectivity. Experimental data illustrated the influence of functional groups on the triterpene skeleton.The MIP was applied to the solid-phase extraction of triterpenoids from a plant extract prior HPLC analysis. However, CHCl3 was replaced by ACN during the washing step in order to suppress non-specific interactions due to polar matrix components. A selective extraction of 18-beta-glycyrrhetinic acid from hydrolyzed extract of liquorice roots was achieved with a good extraction yield (98%). (c) 2007 Elsevier B.V. All rights reserved.